Increased collagen and fluid content of mouse kidneys at 9 months after single or fractionated X radiation

Journal Article · Sun Jul 01 04:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576456· OSTI ID:6599333

Meistrich, M L; Williams, M V; Soranson, J; Fowler, J F; Denekamp, J

Mouse kidneys have been analyzed at sacrifice, 9 months after single-dose and fractionated irradiation, using wet and dry weight, a biochemical determination of hydroxyproline, and quantitation of dilated renal tubules in histological preparations. Dose-response curves have been constructed to determine the sensitivity and precision of the assays and to study the influence of dose fractionation on a variety of radiation responses of the kidney. There was a marked loss of kidney weight, measured either wet or dry, with maximum changes from control values by factors of 3 and 5, respectively. The wet:dry weight ratio increased with X-ray dose, indicating that relative fluid content was increased even 9 months after irradiation. Total collagen content per kidney, determined by a hydroxyproline assay, showed a less marked dose dependence, with a maximum increase of a factor of 1.4. However, hydroxyproline per dry weight increased by a factor of 7, and this ratio proved to be the most sensitive and precise measure of radiation damage.

• Research Organization: Mount Vernon Hospital, Northwood, England
• OSTI ID: 6599333
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 99:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ACUTE IRRADIATION
AMINES
AMINO ACIDS
ANIMALS
AZOLES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBOXYLIC ACIDS
COLLAGEN
DATA
DELAYED RADIATION EFFECTS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROSIS
FRACTIONATED IRRADIATION
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTOLOGY
HYDROXY ACIDS
HYDROXYPROLINE
INFORMATION
IONIZING RADIATIONS
IRRADIATION
KIDNEYS
MAMMALS
MICE
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PROTEINS
PYRROLES
PYRROLIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SCLEROPROTEINS
VERTEBRATES
WEIGHT
X RADIATION