U. v. -enhanced reactivation of u. v. -irradiated herpes virus by primary cultures of rat hepatocytes

Journal Article · Sat Mar 31 23:00:00 EST 1984 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/5.4.495· OSTI ID:6597529

Zurlo, J; Yager, J D ^[1]

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1. Dartmouth Medical School, Hanover, NH (USA)

Carcinogen treatment of cultured mammalian cells prior to infection with u.v.-irradiated virus results in enhanced virus survival and mutagenesis suggesting the induction of SOS-type processes. The development of a primary rat hepatocyte culture system is reported to investigate cellular responses to DNA damage which may be relevant to hepatocarcinogenesis in vivo. Enhanced reactivation of u.v.-irradiated Herpes simplex virus type 1 (HSV-1) occurred in hepatocytes irradiated with u.v. Cultured hepatocytes were pretreated with u.v. at the time of enhanced DNA synthesis. These treatments caused an inhibition followed by a recovery of DNA synthesis. At various times after pretreatment, the hepatocytes were infected with control or u.v.-irradiated HSV-1 at low multiplicity, and virus survival was measured. U.v.-irradiated HSV-1 exhibited the expected two-component survival curve in control or u.v. pretreated hepatocytes. The magnitude of enhanced reactivation of HSV-1 was dependent on the u.v. dose to the hepatocytes, the time of infection following u.v. pretreatment, and the level of DNA synthesis at the time of pretreatment. These results suggest that u.v. treatment of rat hepatocytes causes the induction of SOS-type functions tht may have a role in the initiation of hepatocarcinogenesis.

• OSTI ID: 6597529
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 5:4; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560121 -- Radiation Effects on Cells-- External Source-- (-1987)
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
DISEASES
DNA REPLICATION
ELECTROMAGNETIC RADIATION
HERPES SIMPLEX
HOST-CELL REACTIVATION
INFECTIOUS DISEASES
LIVER CELLS
MAMMALS
MICROORGANISMS
NUCLEIC ACID REPLICATION
PARASITES
PHOTOREACTIVATION
RADIATIONS
RATS
RECOVERY
REPAIR
RODENTS
SKIN DISEASES
SOMATIC CELLS
SURVIVAL CURVES
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
VIRUSES