Double minute chromatin bodies and other chromosome alterations in human myeloid HL-60 leukemia cells susceptible or resistant to induction of differentiation by phorbol-12-myristate-13-acetate

Journal Article · Thu Dec 01 04:00:00 EST 1983 · Cancer Res.; (United States)

OSTI ID:6596409

Au, W W; Callaham, M F; Workman, M L; Huberman, E

An analysis of the chromosomal karyotype of the human promyelocytic HL-60 leukemia cell line and of a number of its sublines that exhibit varying degrees of resistance to induction of differentiation by phorbol-12-myristate-13-acetate was conducted. The HL-60 cell line and the derived sublines contained two consistent marker chromosomes (9p- and t(10;13)), which suggested that they have a common and possibly clonal origin. HL-60 cells that are susceptible to phorbol-12-myristate-13-acetate-induced cell differentiation contained double minute chromatine bodies. The sublines with different degrees of resistance showed a corresponding sequential reduction of double minute chromatin bodies in metaphase cells. This loss of double minute chromatin bodies was not associated with an appearance of homogeneously staining chromosomal regions. Resistant and susceptible HL-60 cell differed also in a number of other chromosomal alteration, including gains or losses involving chromosomes 5, 8, 11, 13, 16, and 17. Thus, it is suggested that acquisition of resistance to phorbol-12-myristate-13-acetate-induced cell differentiation in the HL-60 cells may involve one or more of the above chromosomal changes.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26; W-31109-ENG-38
• OSTI ID: 6596409
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 43; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560306* -- Chemicals Metabolism & Toxicology-- Man-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
ANIMALS
BIOLOGICAL EFFECTS
CARBOXYLIC ACID SALTS
CARCINOGENS
CELL CULTURES
CELL DIFFERENTIATION
CHROMATIN
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DISEASES
ESTERS
HEMIC DISEASES
LEUKEMIA
MAMMALS
MAN
MUTATIONS
NEOPLASMS
ORGANIC COMPOUNDS
PHORBOL ESTERS
PRIMATES
SALTS
VERTEBRATES