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Title: Mechanism of binding of the radiosensitizers metronidazole and misonidazole (RO-07-0582) to bovine and human serum albumin: a proton NMR study

Abstract

High-resolution proton NMR spectra of the radiosensitizer metronidazole and its derivative misonidazole (RO-07-0582) were measured in D/sub 2/O at resonance frequency 60 MHz and interpreted in the aliphatic and aromatic regions. The linewidths of the NMR peaks attributed to individual fragments of nitroimidazole molecules were then analyzed in the presence of bovine and human serum albumin. With increasing concentration of serum albumin, a selectively larger broadening of the lines attributable to the protons of the aliphatic moieties than of those of the imidazole rings was observed for both compounds. This broadening for misonidazole strongly depends on the ionic strength of the solution. The results indicate a specific immobilization of the molecules of both radiosensitizers during their interaction with serum albumin and the involvement of the aliphatic chains of misonidazole and metronidazole as the primary binding sites.

Authors:
; ;
Publication Date:
Research Org.:
Inst. of Chemistry and Physics, Jagiellonska, Poland
OSTI Identifier:
6596387
Alternate Identifier(s):
OSTI ID: 6596387
Resource Type:
Journal Article
Resource Relation:
Journal Name: Radiat. Res.; (United States); Journal Volume: 85:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ALBUMINS; CONFIGURATION INTERACTION; METRONIDAZOLE; NUCLEAR MAGNETIC RESONANCE; MISONIDAZOLE; BLOOD SERUM; CATTLE; HEAVY WATER; MAN; MOLECULAR STRUCTURE; ALCOHOLS; ANIMALS; ANTINEOPLASTIC DRUGS; AZOLES; DOMESTIC ANIMALS; DRUGS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; IMIDAZOLES; MAGNETIC RESONANCE; MAMMALS; NITRO COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXYGEN COMPOUNDS; PRIMATES; PROTEINS; RADIOSENSITIZERS; RESONANCE; RUMINANTS; VERTEBRATES; WATER 560110* -- Radiation Effects on Biochemicals-- (-1987)

Citation Formats

Sulkowska, A., Lubas, B., and Wilczok, T. Mechanism of binding of the radiosensitizers metronidazole and misonidazole (RO-07-0582) to bovine and human serum albumin: a proton NMR study. United States: N. p., 1981. Web. doi:10.2307/3575433.
Sulkowska, A., Lubas, B., & Wilczok, T. Mechanism of binding of the radiosensitizers metronidazole and misonidazole (RO-07-0582) to bovine and human serum albumin: a proton NMR study. United States. doi:10.2307/3575433.
Sulkowska, A., Lubas, B., and Wilczok, T. Thu . "Mechanism of binding of the radiosensitizers metronidazole and misonidazole (RO-07-0582) to bovine and human serum albumin: a proton NMR study". United States. doi:10.2307/3575433.
@article{osti_6596387,
title = {Mechanism of binding of the radiosensitizers metronidazole and misonidazole (RO-07-0582) to bovine and human serum albumin: a proton NMR study},
author = {Sulkowska, A. and Lubas, B. and Wilczok, T.},
abstractNote = {High-resolution proton NMR spectra of the radiosensitizer metronidazole and its derivative misonidazole (RO-07-0582) were measured in D/sub 2/O at resonance frequency 60 MHz and interpreted in the aliphatic and aromatic regions. The linewidths of the NMR peaks attributed to individual fragments of nitroimidazole molecules were then analyzed in the presence of bovine and human serum albumin. With increasing concentration of serum albumin, a selectively larger broadening of the lines attributable to the protons of the aliphatic moieties than of those of the imidazole rings was observed for both compounds. This broadening for misonidazole strongly depends on the ionic strength of the solution. The results indicate a specific immobilization of the molecules of both radiosensitizers during their interaction with serum albumin and the involvement of the aliphatic chains of misonidazole and metronidazole as the primary binding sites.},
doi = {10.2307/3575433},
journal = {Radiat. Res.; (United States)},
number = ,
volume = 85:1,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1981},
month = {Thu Jan 01 00:00:00 EST 1981}
}