Structure, function, and long-term maintenance of the isolated turtle colon
- Brookhaven National Lab., Upton, NY
We describe the 5-day maintenance of sacs of turtle colonic mucosa in enriched bathing solutions. The mean maximum transepithelial potential difference (PD) developed by the sacs in Ringer solution enriched with tissue-culture medium and gassed with 95% air-5% CO/sub 2/ was 126 mV at 24 hours. Lower values were observed in other solutions. The PD of 24-hour sacs was partially or totally inhibited by ouabain, replacement of Na by choline in mucosal bathing fluids, or removal of Ca from serosal bathing fluids. The sacs transported Na in excess of H/sub 2/O forming a dilute mucosal solution. The responses of four different sac preparations (normally oriented or everted, and stripped normally oriented or everted) to long incubation were compared. Stripped normally oriented tissue developed the highest PD and maintained the lowest water content. The morphology of fresh and long-incubated tissue was examined. This investigation demonstrates that the turtle colon can be maintained in vitro for long periods, and it provides information on the morphology and physiology of this tissue.
- OSTI ID:
- 6592584
- Journal Information:
- J. Exp. Zool.; (United States), Vol. 203:3
- Country of Publication:
- United States
- Language:
- English
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CULTURE MEDIA
LARGE INTESTINE
TISSUE CULTURES
MAINTENANCE
CALCIUM
IN VITRO
MORPHOLOGY
MUCOUS MEMBRANES
OUABAIN
PHYSIOLOGY
SODIUM
TISSUES
TURTLES
ALKALI METALS
ALKALINE EARTH METALS
ANIMALS
BODY
CARBOHYDRATES
CARDIOTONICS
DIGESTIVE SYSTEM
DRUGS
ELEMENTS
GASTROINTESTINAL TRACT
GLYCOSIDES
INTESTINES
METALS
ORGANIC COMPOUNDS
ORGANS
REPTILES
STEROIDS
STROPHANTIN
VERTEBRATES
550800* - Morphology
551000 - Physiological Systems
550300 - Cytology