Characterization of ethanol-inducible human liver N-nitrosodimethylamine demethylase
Journal Article
·
· Biochemistry; (United States)
Through the use of monospecific antibodies directed against hepatic cytochrome P-450j, an enzyme induced in rats treated with ethanol or isoniazid, we have purified from human liver the related cytochrome P-450 termed HLj. HLj resembles rat P-450j and P-450 LM3a, the homologous cytochrome in rabbit liver, in its NH/sub 2/-terminal amino acid sequence, in being in highest concentration in liver microsome samples prepared from two patients intoxicated by ethanol and one patient given isoniazid, and in catalyzing the metabolic activation of the procarcinogen N-nitrosodimethylamine. Furthermore, each of nine human liver RNA samples contained a species of mRNA hybridizable to a cloned HLj cDNA. We conclude that HLj is related by structure, function, and some regulator characteristics to rat P-450j and rabbit P-450 LM3a, cytochromes critical for metabolism of several clinically relevant cytotoxic and carcinogenic agents.
- Research Organization:
- Medical College of Virginia, Richmond
- OSTI ID:
- 6592537
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 25:22; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINO ACID SEQUENCE
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBODIES
ANTIMICROBIAL AGENTS
AROMATICS
BENZOPYRENE
BODY
CARBON 14 COMPOUNDS
CARCINOGENS
CONDENSED AROMATICS
CYTOCHROMES
DIGESTIVE SYSTEM
DNA
DRUGS
ENZYMES
ETHANOL
GLANDS
HYDRAZIDES
HYDROCARBONS
HYDROLASES
HYDROXY COMPOUNDS
ISONIAZID
LABELLED COMPOUNDS
LIVER
MAMMALS
MESSENGER-RNA
METABOLIC ACTIVATION
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATIENTS
PIGMENTS
PROTEINS
RABBITS
RADIOASSAY
RATS
RECOMBINANT DNA
RNA
RODENTS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
AMINO ACID SEQUENCE
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBODIES
ANTIMICROBIAL AGENTS
AROMATICS
BENZOPYRENE
BODY
CARBON 14 COMPOUNDS
CARCINOGENS
CONDENSED AROMATICS
CYTOCHROMES
DIGESTIVE SYSTEM
DNA
DRUGS
ENZYMES
ETHANOL
GLANDS
HYDRAZIDES
HYDROCARBONS
HYDROLASES
HYDROXY COMPOUNDS
ISONIAZID
LABELLED COMPOUNDS
LIVER
MAMMALS
MESSENGER-RNA
METABOLIC ACTIVATION
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NITROSO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PATIENTS
PIGMENTS
PROTEINS
RABBITS
RADIOASSAY
RATS
RECOMBINANT DNA
RNA
RODENTS
VERTEBRATES