Effects of controlled gas environments in microbial enhancement of plant protein recovery
Controlled gas environments were maintained by a novel aeration system in solid substrate fermentations for enhanced protein recovery from pressed alfalfa residues. High O/sub 2/ pressures stimulated biomass and enzyme production by an Aspergillus species, isolated from alfalfa, which produced cellulase and pectinase activities in growth-associated metabolism. High CO/sub 2/ pressures also stimulated enzyme production, but had less effect on biomass production, as established from the dissimilation of plant solids. Cellulase and pectinase activities were generally related to protein recoveries. Recoveries were greater than or equal to 50% higher than those obtained by mechanical extraction, with maximum recoveries of greater than or equal to 70% of crude protein contents. Protein not recovered at high cellulase and pectinase activities were believed to be in structurally bound forms not amenable to recovery by nonproteolytic enzymes. Buffering at pH 8 and autoclaving of residues prior to fermentation had little effect on protein recoveries. Controlled gas environments are seen to offer an interesting potential for optimizing industrial fermentation processes for the production of microbial enzymes.
- Research Organization:
- Massachusetts Agric Exp Stn, Univ Massachusetts, Amherst, MA 01003 USA
- OSTI ID:
- 6590818
- Journal Information:
- J. Food Biochem.; (United States), Vol. 3:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
SINGLE CELL PROTEIN
PRODUCTION
ALFALFA
ASPERGILLUS
CARBON DIOXIDE
CELLULASE
OXYGEN
PLANTS
RESIDUES
YIELDS
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
ELEMENTS
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
LEGUMINOSAE
NONMETALS
O-GLYCOSYL HYDROLASES
OXIDES
OXYGEN COMPOUNDS
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
550700 - Microbiology