Production of SCP and cellulase by Aspergillus terreus from bagasse substrate
The fermentation of 1.0% untreated bagasse under optimum cultural and nutritional conditions with Aspergillus terreus GN1 indicated that the maximum rate of protein and cellulase production could be obtained during three days of submerged fermentation. Even though 16.4% protein recovery, 0.55 units CMCase/mL, and 0.027 FPase units/mL were obtained on the seventh day, the rates of increase in protein recovery and cellulase production were slower than those obtained up to three days, which were 14.3% protein recovery, 0.45 units cMCase/mL, and 0.019 units FPase/mL. There was an initial lag in the utilization of cellulose up to two days due to the utilization of the water-soluble carbohydrate present in untreated bagasse. Cellulose utilization and water-soluble carbohydrate content during fermentation were correlated with protein recovery and enzyme production. The protein and cellulase production during three days fermentation with 1.0% untreated and treated bagasse were compared and the protein content of the total biomass was calculated into constituent protein contributed by the fungal mycelium and the undegraded bagasse. The total biomass recovered with untreated and treated bagasse was 1020 and 820 mg/g bagasse substrate, respectively, and contained 14.3 and 20.6% crude protein, respectively. The contribution of fungal biomass and undegraded bagasse was 309 and 711, and 373 and 447 mg/g untreated and treated bagasse substrates, respectively. In an 8-L-flask trial during three days of fermentation, the recovery of SCP and cellulase were 66 g and 32,400 units (Sigma) for treated bagasse and 82 g and 8200 units (Sigma) for untreated bagasse, respectively. (Refs. 18).
- Research Organization:
- Dairy Microbiology Div, National Dairy Research Inst, Karnal - 132001 (Haryara), India
- OSTI ID:
- 6060000
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 24; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
ASPERGILLUS
BAGASSE
BIOCONVERSION
CARBOHYDRATES
CELLULASE
CELLULOSE
ENZYMES
FERMENTATION
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC WASTES
PLANTS
POLYSACCHARIDES
SACCHARIDES
SINGLE CELL PROTEIN
SUBSTRATES
WASTES