Interferon alpha/beta synthesis during acute graft-versus-host disease
Graft-versus-host disease (GVHD) is a major obstacle to successful bone marrow transplantation. The role of interferon (IFN) in GVHD is currently unclear. We have previously shown that interferon (IFN) is produced in vitro by alloantigen-stimulated murine bone marrow cells. This study was initiated to examine whether IFN production occurs in vivo during GVHD. Lethally irradiated mice were given bone marrow (10(7)) and/or spleen cells (2 X 10(7)) from either allogeneic or syngeneic mice. Mice given allogeneic spleen cells or bone marrow and spleen cells showed signs of GVHD within 10 days and usually died within 20 days of transplantation. Mice undergoing GVHD were found to have significant levels of IFN activity in their sera. Serum IFN was detected early (day 3) after transplantation with optimal IFN activity (greater than or equal to 80 units) occurring at 5-6 days. The IFN activity present in the sera obtained from mice with GVHD was identified as IFN alpha/beta by using specific antisera against IFN alpha/beta and IFN gamma. In contrast, irradiated mice given T-cell-depleted allogeneic bone marrow and spleen cells failed to develop GVHD and had no detectable serum IFN activity. Irradiated mice given syngeneic bone marrow and spleen cells or only allogeneic bone marrow cells did not develop GVHD and did not produce detectable IFN activity in their sera. These results show that serum IFN activity correlates well with GVHD and opens for speculation the possibility that IFN may be involved in the pathogenesis associated with GVHD.
- Research Organization:
- Univ. of Texas Medical Branch, Galveston
- OSTI ID:
- 6585174
- Journal Information:
- Transplantation; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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BONE MARROW CELLS
TRANSPLANTS
INTERFERON
BIOSYNTHESIS
GRAFT-HOST REACTION
IMMUNOSUPPRESSION
LETHAL IRRADIATION
LYMPHOCYTES
MICE
RADIATION CHIMERAS
SPLEEN CELLS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
IRRADIATION
LEUKOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MITOGENS
MOSAICISM
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals