Regulation of the colony-stimulating activity produced by a murine marrow-derived cell line (H-1)

Journal Article · Mon Mar 01 00:00:00 EST 1982 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.79.5.1545· OSTI ID:6583625

Garnett, H M ^[1]; Cronkite, E P; Harigaya, K

---

1. Brookhaven National Lab., Upton, NY

The production of molecular species that stimulate growth of granulocyte or macrophage colonies (GM-CSF) by the fibroblastoid H-1 cell line is unaffected by either native or iron-saturated lactoferrin, although some inhibition is detected with 10 ..mu..M prostaglandin E/sub 1/. The H-1 GM-CSF is able to support the formation of macrophage, neutrophil, and mixed colonies. This inhibitory effect is observed irrespective of the presence of an additional agar layer between the feeder cells and plated bone marrow cells, implying that diffusable substances are involved. Addition of indomethacin (10 ..mu..M) to feeder layers derived from 2.5 x 10/sup 3/ H-1 cells increases the number of GM-CFU/sub c/ detected to 50% of that seen with conditioned medium alone. This result suggests that released prostaglandin may be responsible for some, but not all, of the observed inhibition of colony formation. In the presence of the H-1 feeder layers, only macrophage colonies are detected and hence it appears that the H-1 cells produce, in addition to prostaglandin, a diffusible inhibitory substance that preferentially inhibits granulopoiesis.

• DOE Contract Number: AC02-76CH00016
• OSTI ID: 6583625
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 79
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COLONY FORMATION
STIMULATION
LACTOFERRIN
BIOLOGICAL EFFECTS
PROSTAGLANDINS
INHIBITION
LEUKOCYTES
LEUKOPOIESIS
MACROPHAGES
MICE
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
GLOBULINS
GLUCOPROTEINS
MAMMALS
MATERIALS
METALLOPROTEINS
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
550300* - Cytology