Enhancement of the grafting efficiency of transplanted marrow cells by preincubation with interleukin-3 and granulocyte-macrophage colony-stimulating factor
To improve the grafting efficiency of transplanted murine hematopoietic progenitors, we briefly preincubated mouse bone marrow cells with interleukin-3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) ex vivo before their transplantation into irradiated recipients. This treatment was translated into an increase in the seeding efficiency of colony-forming unit-spleen (CFU-S) and CFU-GM after transplantation. Not only was the concentration of CFU-S in the tibia increased 2 and 24 hours after transplantation, but the total cell number and CFU-S and CFU-GM concentrations were persistently higher in IL-3- and GM-CSF-treated groups 1 to 3 weeks after transplantation. In addition, the survival of animals as a function of transplanted cell number was persistently higher in IL-3- and GM-CSF-treated groups compared with controls. The data indicate that the pretreatment of marrow cells with IL-3 and GM-CSF before transplantation increases the seeding efficiency of hematopoietic stem cells and probably other progenitor cells after transplantation. This increased efficiency may be mediated by upward modulation of homing receptors. Therefore, ex vivo preincubation of donor marrow cells with IL-3 and GM-CSF may be a useful tactic in bone marrow transplantation.
- OSTI ID:
- 5573948
- Journal Information:
- Blood (Journal of Hematology); (USA), Journal Name: Blood (Journal of Hematology); (USA) Vol. 77:7; ISSN BLOOA; ISSN 0006-4971
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BONE MARROW CELLS
CELL CULTURES
CLONE CELLS
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
GLYCOPROTEINS
GROWTH FACTORS
IRRADIATION
LYMPHOKINES
MAMMALS
MEDICINE
MICE
MITOGENS
ORGANIC COMPOUNDS
PROTEINS
RADIOSENSITIVITY EFFECTS
RODENTS
SOMATIC CELLS
SPLENECTOMY
STEM CELLS
SURGERY
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION