Studies on the chalcone synthase gene of two higher plants: petroselinum hortense and matthiola incana

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Prog. Clin. Biol. Res.; (United States)

OSTI ID:6583079

Hemleben, V; Frey, M; Rall, S; Koch, M; Kittel, M; Kreuzaler, F; Ragg, H; Fautz, E; Hahlbrock, K

Two higher plant systems are presented which allow to study coordinated gene expression of the light-induced metabolic pathway of flavonoid biosynthesis: tissue culture cells of Petroselinum hortense (Apiaceae) and different developmental stages of various genotypes of Matthiola incana (Brassicaceae). The gene structure of the chalcone synthase is mainly studied. A cDNA clone (pLF56) of parsley has been constructed and characterized conferring the chalcone synthase gene sequence. Strong cross hybridization between the parsley cDNA and Matthiola DNA allowed to identify a HindIII fragment (6000 bp) identical in size for parsley and different Matthiola wild type lines and a mutant line.

• Research Organization: University of Tuebingen, Institute of Biology II, FRG
• OSTI ID: 6583079
• Journal Information: Prog. Clin. Biol. Res.; (United States), Vol. 85 Pt B
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FLAVONES
BIOSYNTHESIS
PLANTS
CLONING
BIOLOGICAL PATHWAYS
DNA
ENZYME ACTIVITY
GENOTYPE
HYBRIDIZATION
MUTANTS
TISSUE CULTURES
VISIBLE RADIATION
ELECTROMAGNETIC RADIATION
FLAVENOIDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RADIATIONS
SYNTHESIS
550400* - Genetics