The Sorghum (Sorghum bicolor) Brown Midrib 30 Gene Encodes a Chalcone Isomerase Required for Cell Wall Lignification

Tetreault, Hannah M.; Gries, Tammy; Liu, Sarah; Toy, John; Xin, Zhanguo; Vermerris, Wilfred; Ralph, John; Funnell-Harris, Deanna L.; Sattler, Scott E.

doi:10.3389/fpls.2021.732307

The Sorghum (Sorghum bicolor) Brown Midrib 30 Gene Encodes a Chalcone Isomerase Required for Cell Wall Lignification

Journal Article · Thu Dec 02 00:00:00 EST 2021 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2021.732307· OSTI ID:1846259

Tetreault, Hannah M. ^[1]; Gries, Tammy ^[2]; Liu, Sarah ^[3]; Toy, John ^[2]; Xin, Zhanguo ^[4]; Vermerris, Wilfred ^[5]; Ralph, John ^[3]; Funnell-Harris, Deanna L. ^[2]; Sattler, Scott E. ^[2]

US Dept. of Agriculture (USDA), Lincoln, NE (United States). Agricultural Research Service; Univ. of Nebraska, Lincoln, NE (United States); United States Department of Agriculture, Lincoln, NE
US Dept. of Agriculture (USDA), Lincoln, NE (United States). Agricultural Research Service; Univ. of Nebraska, Lincoln, NE (United States)
Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); Univ. of Wisconsin, Madison, WI (United States)
US Dept. of Agriculture (USDA), Lubbock, TX (United States). Agricultural Research Service
Univ. of Florida, Gainesville, FL (United States)

In sorghum (Sorghum bicolor) and other C₄ grasses, brown midrib (bmr) mutants have long been associated with plants impaired in their ability to synthesize lignin. The brown midrib 30 (Bmr30) gene, identified using a bulk segregant analysis and next-generation sequencing, was determined to encode a chalcone isomerase (CHI). Two independent mutations within this gene confirmed that loss of its function was responsible for the brown leaf midrib phenotype and reduced lignin concentration. Loss of the Bmr30 gene function, as shown by histochemical staining of leaf midrib and stalk sections, resulted in altered cell wall composition. In the bmr30 mutants, CHI activity was drastically reduced, and the accumulation of total flavonoids and total anthocyanins was impaired, which is consistent with its function in flavonoid biosynthesis. The level of the flavone lignin monomer tricin was reduced 20-fold in the stem relative to wild type, and to undetectable levels in the leaf tissue of the mutants. The bmr30 mutant, therefore, harbors a mutation in a phenylpropanoid biosynthetic gene that is key to the interconnection between flavonoids and monolignols, both of which are utilized for lignin synthesis in the grasses.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Organization:: USDA; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FG02-07ER64458; SC0018409

OSTI ID:: 1846259

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 12; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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