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Title: Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content

Abstract

Lignin is a phenolic heteropolymer that is deposited in secondary-thickened cell walls, where it provides mechanical strength. A recent structural characterization of cell walls from monocot species showed that the flavone tricin is part of the native lignin polymer, where it is hypothesized to initiate lignin chains. In this study, we investigated the consequences of altered tricin levels on lignin structure and cell wall recalcitrance by phenolic profiling, nuclear magnetic resonance, and saccharification assays of the naturally silenced maize (Zea mays) C2-Idf (inhibitor diffuse) mutant, defective in the CHALCONE SYNTHASE Colorless2 (C2) gene. We show that the C2-Idf mutant produces highly reduced levels of apigenin- and tricin-related flavonoids, resulting in a strongly reduced incorporation of tricin into the lignin polymer. Moreover, the lignin was enriched in β-β and β-5 units, lending support to the contention that tricin acts to initiate lignin chains and that, in the absence of tricin, more monolignol dimerization reactions occur. In addition, the C2-Idf mutation resulted in strikingly higher Klason lignin levels in the leaves. As a consequence, the leaves of C2-Idf mutants had significantly reduced saccharification efficiencies compared with those of control plants. These findings are instructive for lignin engineering strategies to improve biomass processingmore » and biochemical production.« less

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [1]; ORCiD logo [4]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [5]; ORCiD logo [1]
  1. Center for Plant Systems Biology, VIB (Belgium); Ghent University (Belgium). Department of Plant Biotechnology and Bioinformatics
  2. Univ. of Wisconsin, Madison, WI (United States). Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute and Department of Biological System Engineering
  3. Center for Plant Systems Biology, VIB (Belgium); Ghent University (Belgium). Department of Plant Biotechnology and Bioinformatics; University of Sao Paulo, Butanta, Sao Paulo (Brazil)
  4. Univ. of Wisconsin, Madison, WI (United States). Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute and Department of Biochemistry
  5. Univ. of Wisconsin, Madison, WI (United States). Department of Energy Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, Department of Biological System Engineering and Department of Biochemistry
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1427679
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Volume: 173; Journal Issue: 2; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Eloy, Nubia B., Voorend, Wannes, Lan, Wu, Saleme, Marina de Lyra Soriano, Cesarino, Igor, Vanholme, Ruben, Smith, Rebecca A., Goeminne, Geert, Pallidis, Andreas, Morreel, Kris, Nicomedes, Jose, Ralph, John, and Boerjan, Wout. Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content. United States: N. p., 2016. Web. doi:10.1104/pp.16.01108.
Eloy, Nubia B., Voorend, Wannes, Lan, Wu, Saleme, Marina de Lyra Soriano, Cesarino, Igor, Vanholme, Ruben, Smith, Rebecca A., Goeminne, Geert, Pallidis, Andreas, Morreel, Kris, Nicomedes, Jose, Ralph, John, & Boerjan, Wout. Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content. United States. doi:10.1104/pp.16.01108.
Eloy, Nubia B., Voorend, Wannes, Lan, Wu, Saleme, Marina de Lyra Soriano, Cesarino, Igor, Vanholme, Ruben, Smith, Rebecca A., Goeminne, Geert, Pallidis, Andreas, Morreel, Kris, Nicomedes, Jose, Ralph, John, and Boerjan, Wout. Fri . "Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content". United States. doi:10.1104/pp.16.01108. https://www.osti.gov/servlets/purl/1427679.
@article{osti_1427679,
title = {Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content},
author = {Eloy, Nubia B. and Voorend, Wannes and Lan, Wu and Saleme, Marina de Lyra Soriano and Cesarino, Igor and Vanholme, Ruben and Smith, Rebecca A. and Goeminne, Geert and Pallidis, Andreas and Morreel, Kris and Nicomedes, Jose and Ralph, John and Boerjan, Wout},
abstractNote = {Lignin is a phenolic heteropolymer that is deposited in secondary-thickened cell walls, where it provides mechanical strength. A recent structural characterization of cell walls from monocot species showed that the flavone tricin is part of the native lignin polymer, where it is hypothesized to initiate lignin chains. In this study, we investigated the consequences of altered tricin levels on lignin structure and cell wall recalcitrance by phenolic profiling, nuclear magnetic resonance, and saccharification assays of the naturally silenced maize (Zea mays) C2-Idf (inhibitor diffuse) mutant, defective in the CHALCONE SYNTHASE Colorless2 (C2) gene. We show that the C2-Idf mutant produces highly reduced levels of apigenin- and tricin-related flavonoids, resulting in a strongly reduced incorporation of tricin into the lignin polymer. Moreover, the lignin was enriched in β-β and β-5 units, lending support to the contention that tricin acts to initiate lignin chains and that, in the absence of tricin, more monolignol dimerization reactions occur. In addition, the C2-Idf mutation resulted in strikingly higher Klason lignin levels in the leaves. As a consequence, the leaves of C2-Idf mutants had significantly reduced saccharification efficiencies compared with those of control plants. These findings are instructive for lignin engineering strategies to improve biomass processing and biochemical production.},
doi = {10.1104/pp.16.01108},
journal = {Plant Physiology (Bethesda)},
issn = {0032-0889},
number = 2,
volume = 173,
place = {United States},
year = {2016},
month = {12}
}

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Works referencing / citing this record:

The lignin toolbox of the model grass Setaria viridis
journal, June 2019

  • Ferreira, Sávio Siqueira; Simões, Marcella Siqueira; Carvalho, Gabriel Garon
  • Plant Molecular Biology, Vol. 101, Issue 3
  • DOI: 10.1007/s11103-019-00897-9

The lignin toolbox of the model grass Setaria viridis
journal, June 2019

  • Ferreira, Sávio Siqueira; Simões, Marcella Siqueira; Carvalho, Gabriel Garon
  • Plant Molecular Biology, Vol. 101, Issue 3
  • DOI: 10.1007/s11103-019-00897-9

Color for Life: Biosynthesis and Distribution of Phenolic Compounds in Pepper (Capsicum annuum)
text, January 2019

  • Carvalho Lemos, Virginia; Reimer, Julia Jessica; Wormit, Alexandra
  • RWTH Aachen University
  • DOI: 10.18154/rwth-2019-05090

Lignins: Biosynthesis and Biological Functions in Plants
journal, January 2018

  • Liu, Qingquan; Luo, Le; Zheng, Luqing
  • International Journal of Molecular Sciences, Vol. 19, Issue 2
  • DOI: 10.3390/ijms19020335