Retardation of senescence in red clover leaf discs by a new antiozonant, N-(2-(2-oxo-2-imidazolidinyl)ethyl)-N'-phenylurea
Dark-induced senescence in leaf discs from O/sub 3/-sensitive red clover trifoliates (Trifolium pratense L. cv. Pennscott) was markedly retarded by treatment with N-(2-(2-oxo-1-imidazolidinyl))-ethyl-N'-phenylurea (EDU). EDU also protects against acute and chronic foliar O/sub 3/ injury when sprayed on intact leaves or supplied to the plants through soil application. Senescence retardation was measured by time-dependent analyses of chlorophyll, protein, and RNA in discs floated on aqueous EDU solutions ranging from 0 to 500 micrograms per milliliter EDU. Chlorophyll degradation, total protein, and nucleic acids were followed over 10-day test periods. EDU at 500 micrograms per milliliter (50 milligrams per pot), a concentration known to provide optimal protection to intact leaves against O/sub 3/ injury, was most effective in preventing chlorosis and in maintaining high concentrations of protein and RNA in the discs. In discs treated with 500 micrograms per milliliter EDU 90% of the chlorophyll was retained after 10 days in the dark. In contrast, lower concentrations (0, 125, and 250 micrograms per milliliter) showed the complete loss of chlorophyll or an intermediate retardation. The intermediate concentrations were similarly less effective in maintaining protein and RNA levels in the dark stressed leaf discs. It is suggested that EDU retards senescence and mitigates O/sub 3/ injury through the induction of specific free radical scavenging enzymes and in sustaining RNA and protein synthesis.
- Research Organization:
- Department of Agriculture, Science and Education Administration-Agricultural Research, Beltsville, MD
- OSTI ID:
- 6582967
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 67:2; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Patterns of ethylene production in senescing leaves
Efficacy of the antioxidant ethylene-diurea (EDU) compared to carboxin and benomyl in reducing yield losses from ozone in navy bean
Related Subjects
560303 -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOASSAY
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL STRESS
CARBOXYLIC ACIDS
CHEMISTRY
CHLOROPHYLL
CLOVER
ENZYME ACTIVITY
ENZYMES
ESTERASES
FOLIAR UPTAKE
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROLASES
LEAVES
LEGUMINOSAE
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OZONE
PHOSPHODIESTERASES
PHYTOCHROMES
PIGMENTS
PLANT GROWTH
PLANTS
PORPHYRINS
PROTEINS
QUANTITY RATIO
RESPONSE MODIFYING FACTORS
RNA-ASE
SENSITIVITY
TIME DEPENDENCE
UPTAKE