Iron absorption in man calculated from erythrocyte incorporation of the stable isotope iron-54 determined by fast atom bombardment mass spectrometry

Journal Article · Fri Jul 01 04:00:00 EDT 1988 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(88)90424-1· OSTI ID:6582597

Lehmann, W D; Fischer, R; Heinrich, H C

The methodology of precise isotope abundance determinations of erythrocyte iron by fast atom bombardment mass spectrometry and signal averaging is established. For the determination of the 54Fe/56Fe ratio a relative precision of 0.5% and an absolute precision of 0.03% is achieved. After oral loading with 54Fe-enriched samples in the range between 5 and 25 mg per subject, the 14-day erythrocyte incorporation of 54Fe has been determined in five individuals, namely, two adults, two children, and one infant. In the two adults, the oral dose of 54Fe was simultaneously labeled with a trace amount of carrier-free 59Fe. In these double-isotope loading tests, a good agreement was observed between the absorption data determined on the basis of whole body retention of 59Fe and on the basis of the 54Fe erythrocyte incorporation. The stable isotope methodology applied allows measurement of the iron absorption using highly enriched 54Fe at a dose of 25 mg for an adult or at a dose of 5 mg for infants of about 1 year of age.

• Research Organization: Univ. Hospital Eppendorf, Hamburg (Germany, F.R.)
• OSTI ID: 6582597
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 172:1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ERYTHROCYTES
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
IRON
IRON 54
IRON 56
IRON 59
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPE RATIO
ISOTOPES
MASS SPECTROSCOPY
MATERIALS
METABOLISM
METALS
NUCLEI
RADIOISOTOPES
RETENTION
SPECTROSCOPY
STABLE ISOTOPES
TRACER TECHNIQUES
TRANSITION ELEMENTS
UPTAKE