A rapid assay for evaluation of iron-chelating agents in rats

Journal Article · Thu Oct 01 04:00:00 EDT 1981 · Blood; (United States)

OSTI ID:5369219

Pippard, M J; Johnson, D K; Finch, C A

The animal assay of potential new iron-chelating agents is at present dependent on cumbersome and imprecise iron balance studies in hypertransfused rodents. We report the development of a radioisotope assay in intact rats based on the transient labeling by ferritin 59Fe of the main source of chelatable iron within hepatocytes. The isotope was maximally available to chelators during the first 6 hr after its injection, nearly all the excretion being in the bile. The bile 59Fe/total iron ratio was independent of both the chelator and its dose. However, in iron-loaded rats, the ratio was reduced, and the isotope excretion was a less sensitive measure of intrahepatic chelation. In the proposed assay, test chelators were given to normal rats 2 hr after an intravenous injection of 59Fe-ferritin. Four hours later, the radioiron in the liver and in the gut gave a sensitive measure of the mobilization of hepatic iron to the bile. In addition, chemical iron determinations identified a small alternative source of urinary chelate with agents known to promote urine excretion in man. The assay gave a rapid and precise screen for chelators given by parenteral and oral routes.

• OSTI ID: 5369219
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 58:4; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550601 -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BILE
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CHELATING AGENTS
CHEMISTRY
COMPLEXES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
EVEN-ODD NUCLEI
FERRITIN
GASTROINTESTINAL TRACT
GLANDS
INTERMEDIATE MASS NUCLEI
IRON 59
IRON COMPLEXES
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIVER
MAMMALS
MATERIALS
METALLOPROTEINS
NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOISOTOPES
RATS
RODENTS
TRANSITION ELEMENT COMPLEXES
VERTEBRATES