Cell-mediated mutagenesis and tumor-initiating activity of the ubiquitous polycyclic hydrocarbon, cyclopenta(c,d)pyrene

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/3.7.763· OSTI ID:6578494

Raveh, D; Slaga, T J; Huberman, E

The ubiquitous polycyclic aromatic hydrocarbon (PAH), cyclopenta(c,d) (CPP), was tested to determine its mutagenicity for 6-thioguanine and ouabain resistance in Chinese hamster V79 cells and its tumor-initiating activity in the skin of the tumor susceptible Sencar mice. The potent carcinogen/mutagen, benzo(a)pyrene (BP), was included for comparison. Inasmuch as V79 cells do not metabolize PAHs, mutagenesis was tested both in the presence and in the absence of X-irradiated golden hamster embryo fibroblasts capable of metabolizing PAH. Neither CPP nor BP showed mutagenicity for V79 cells in the absence of the embryo cells. In the presence of these cells (in the cell-mediated assay) both PAHs elicited, in a dose dependent manner, a cytotoxic and mutagenic response in V79 cells, CPP was however less active than BP in inducing both of these responses. CPP was also active in initiating skin tumors in a dose dependent manner in the Sencar mice tumors in a dose dependent manner in the Sencar mice yielding tumors in approx. 60% of the mice at 200 ..mu..g, the highest dose tested. BP was more efficient in tumor initiation and yielded a similar response with 10 ..mu..g. These results indicate that CPP and BP elicit, in the cell mediated assay, a mutagenic response similar to the activity of these PAH in the skin of Sencar mice.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6578494
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 3:7; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
HAMSTERS
HYDROCARBONS
MAMMALS
MICE
MUTAGENESIS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
RODENTS
SKIN
SOMATIC CELLS
VERTEBRATES