Cell-mediated mutagenicity in Chinese hamster V79 cells of dibenzopyrenes and their bay-region fluorine-substituted derivatives
Journal Article
·
· Cancer Res.; (United States)
OSTI ID:6378954
- Oak Ridge National Lab., TN
The polycyclic aromatic hydrocarbons dibenzo(a,i)pyrene and dibenzo(a,h)pyrene, each of which possesses two bay regions, and their bay-region difluorinated derivatives were tested for mutagenicity for ouabain and 6-thioguanine resistance in Chinese hamster V79 cells. Since V79 cells do not metabolize polycyclic aromatic hydrocarbons, mutagenesis was tested in both the presence and the absence of golden hamster embryo cells capable of metabolizing polycyclic aromatic hydrocarbons. Neither of the dibenzopyrenes nor their fluorinated derivatives were mutagenic in the absence of the golden hamster embryo cells. In the presence of these cells (cell-mediated assay), both dibenzopyrenes were mutagenic, whereas the difluorinated derivatives, 2,10-difluorodibenzo(a,i)pyrene and 3,10-difluorobenzo(a,h)pyrene, either were inactive or exhibited (on a dose basis) a weak response. However, the mutagenicity of the dibenzopyrenes was eliminated when they were coincubated with 7,8-benzoflavone, a mixed-function oxidase inhibitor. The results suggest that metabolic oxidation of these polycyclic aromatic hydrocarbons at the bay region (presumably to diol-epoxides) is required for a mutagenic response in the cell-mediated assay.
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6378954
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 42; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550300 -- Cytology
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
AROMATICS
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CULTURES
CONDENSED AROMATICS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EMBRYONIC CELLS
GLYCOSIDES
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
METABOLISM
MUTAGENESIS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OUABAIN
PURINES
STROPHANTHINS
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMAL CELLS
AROMATICS
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CULTURES
CONDENSED AROMATICS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EMBRYONIC CELLS
GLYCOSIDES
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
METABOLISM
MUTAGENESIS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OUABAIN
PURINES
STROPHANTHINS