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Title: Nucleotide sequence analysis of a candidate gene for ataxia-telangiectasia group D (ATDC)

Abstract

A radioresistant cell clone (1B3) was previously isolated after transfection of an ataxia-telangiectasia (AT) group D cell line with a human cosmid library. A cosmid rescued from the integration site in 1B3 contained human DNA from chromosome position 11q23, the same region shown by both genetic linkage and chromosome transfer to contain the genes for AT complementation groups A/B, C, and D. A gene within the cosmid (ATDC) was found to produce mRNAs of different sizes. A cDNA for one of the most abundant mRNAs (3.0 kb) was isolated from a HeLa cell library. In the present study, the authors sequenced the 3.0-kb cDNA and the surrounding intron DNA in the cosmids. They used polymerase chain reaction, with primers in the introns, to confirm the number of exons and to analyze DNA from AT group D cells for mutations within this gene. Although no mutations were found, they do not rule out the possibility that mutations may be present within the regulatory sequences or coding sequences found in other mRNAs specific for this gene. From the sequence analysis, they found that the ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and anmore » adjacent leucine zipper motif. These proteins have been proposed to form homo- or hetero-dimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation. 36 refs., 5 figs., 2 tabs.« less

Authors:
; ; ;  [1]
  1. Univ. of California, San Francisco, CA (United States)
Publication Date:
OSTI Identifier:
6574008
DOE Contract Number:  
AC03-76SF01012
Resource Type:
Journal Article
Journal Name:
Genomics; (United States)
Additional Journal Information:
Journal Volume: 19:1; Journal ID: ISSN 0888-7543
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CEREBELLUM; HEREDITARY DISEASES; GENES; DNA SEQUENCING; DNA-CLONING; TRANSCRIPTION FACTORS; BIOTECHNOLOGY; BODY; BRAIN; CENTRAL NERVOUS SYSTEM; CLONING; DISEASES; DNA HYBRIDIZATION; GENETIC ENGINEERING; HYBRIDIZATION; NERVOUS SYSTEM; NUCLEIC ACID HYBRIDIZATION; ORGANS; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics

Citation Formats

Leonhardt, E A, Kapp, L N, Young, B R, and Murnane, J P. Nucleotide sequence analysis of a candidate gene for ataxia-telangiectasia group D (ATDC). United States: N. p., 1994. Web. doi:10.1006/geno.1994.1022.
Leonhardt, E A, Kapp, L N, Young, B R, & Murnane, J P. Nucleotide sequence analysis of a candidate gene for ataxia-telangiectasia group D (ATDC). United States. https://doi.org/10.1006/geno.1994.1022
Leonhardt, E A, Kapp, L N, Young, B R, and Murnane, J P. 1994. "Nucleotide sequence analysis of a candidate gene for ataxia-telangiectasia group D (ATDC)". United States. https://doi.org/10.1006/geno.1994.1022.
@article{osti_6574008,
title = {Nucleotide sequence analysis of a candidate gene for ataxia-telangiectasia group D (ATDC)},
author = {Leonhardt, E A and Kapp, L N and Young, B R and Murnane, J P},
abstractNote = {A radioresistant cell clone (1B3) was previously isolated after transfection of an ataxia-telangiectasia (AT) group D cell line with a human cosmid library. A cosmid rescued from the integration site in 1B3 contained human DNA from chromosome position 11q23, the same region shown by both genetic linkage and chromosome transfer to contain the genes for AT complementation groups A/B, C, and D. A gene within the cosmid (ATDC) was found to produce mRNAs of different sizes. A cDNA for one of the most abundant mRNAs (3.0 kb) was isolated from a HeLa cell library. In the present study, the authors sequenced the 3.0-kb cDNA and the surrounding intron DNA in the cosmids. They used polymerase chain reaction, with primers in the introns, to confirm the number of exons and to analyze DNA from AT group D cells for mutations within this gene. Although no mutations were found, they do not rule out the possibility that mutations may be present within the regulatory sequences or coding sequences found in other mRNAs specific for this gene. From the sequence analysis, they found that the ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or hetero-dimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation. 36 refs., 5 figs., 2 tabs.},
doi = {10.1006/geno.1994.1022},
url = {https://www.osti.gov/biblio/6574008}, journal = {Genomics; (United States)},
issn = {0888-7543},
number = ,
volume = 19:1,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1994},
month = {Sat Jan 01 00:00:00 EST 1994}
}