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Swelling, NEM, and A23187 activate Cl(-)-dependent K+ transport in high-K+ sheep red cells

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:6570729
;
In low K+ (LK) sheep red cells a significant fraction of the total ouabain-resistant (OR) K+ flux is inhibited when Cl- is replaced by other anions of the Hofmeister series except Br- (Cl(-)-dependent K+ flux). In contrast, high K+ (HK) sheep red cells in isosmotic media did not possess any significant OR Cl(-)-dependent K+ flux when Cl- was replaced by NO/sub 3/- or I-. However, exposure to hyposmotic solutions, treatment with the sulfhydryl (SH) group reagent N-ethylmaleimide (NEM) or with the bivalent metal ion (Me2+) ionophore A23187 in absence of external Me2+ caused a significant activation of Cl(-)-dependent K+ transport as measured with Rb+ as K+ congener. There was no Cl(-)-dependent Rb+ flux in A23187-treated cells when Mn2+, Mg2+, and Ca2+ were present at 1 mM concentrations, suggesting that cellular accumulation of these Me2+ is inhibitory. Similar to LK red cells, HK red cells failed to respond to A23187 when pretreated with NEM supporting the hypothesis proposed recently of a common mechanism of Cl(-)-dependent K+ transport activation. The magnitudes of the Cl(-)-dependent Rb+ fluxes in HK cells were much smaller than those elicited by identical treatments in LK red cells, and the effect of all interventions was not due to the presence of reticulocytes known to possess Cl(-)-dependent K+ transport.
Research Organization:
Wright State Univ., Dayton, OH
OSTI ID:
6570729
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

550500 -- Metabolism
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
ALKALI METALS
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
ANIONS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BROMIDES
BROMINE COMPOUNDS
CALCIUM COMPOUNDS
CATIONS
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
DOMESTIC ANIMALS
ELEMENTS
ERYTHROCYTES
HALIDES
HALOGEN COMPOUNDS
IONS
MAGNESIUM COMPOUNDS
MAMMALS
MANGANESE COMPOUNDS
MATERIALS
MEMBRANE TRANSPORT
METALS
NITRATES
NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
POTASSIUM COMPOUNDS
RESPONSE MODIFYING FACTORS
RUBIDIUM
RUMINANTS
SHEEP
TRANSITION ELEMENT COMPOUNDS
VALENCE
VERTEBRATES