Changes in cellular contents of monovalent cations, chloride and water in hyposmotically swollen control and N-ethylmaleimide-treated low K sheep erythrocytes

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5372954

Bryant, R; Delpire, E; Lauf, P K ^[1]

---

1. Wright State Univ., Dayton, OH (United States)

Low K (LK) sheep red blood cells (SRBC) respond to hyposmotic swelling or treatment with N-ethylmaleimide (NEM) with an increased K:Cl cotransport. Little is known about Cl and water fluxes accompanying K. Thus, the authors measured cellular K, Na, Cl and water (CW) per kilogram dry cell solids (DCS) in control and NEM-pretreated LK SRBC hyposmotically swollen and maintained at 37C for up to 7 hrs in hyposmotic NaCl or NaNO{sub 3} as incubation beyond 7 hrs increased Na content in both. Thus, swollen LK SRBC lose both K and Cl in equal amounts. K loss in NO{sub 3} was much reduced. In NEM-treated, swollen cells, K and Cl losses were stimulated 3- and 2-fold, respectively, suggesting that NEM stimulated K:Cl cotransport in addition to swelling. However, the effect of NEM on Cl was less than on K transport. The tonicity of fluid leaving swollen cells was close to isotonic while that from NEM-treated cells was hyperosmotic, relative to the external solutions. Hence, it is possible to measure net K and Cl movements in LK SRBC in the absence of anion exchange inhibitors.

• OSTI ID: 5372954
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METALS
ANIMALS
ANIONS
ANTIMITOTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CATIONS
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
DOMESTIC ANIMALS
DRUGS
ELEMENTS
ERYTHROCYTES
HALIDES
HALOGEN COMPOUNDS
HYDROGEN COMPOUNDS
IMIDES
ION EXCHANGE
IONS
MAMMALS
MATERIALS
MEMBRANE TRANSPORT
METALS
MORPHOLOGICAL CHANGES
NEM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
POTASSIUM
RADIOSENSITIZERS
RUMINANTS
SHEEP
SODIUM
VERTEBRATES
WATER