Relationship between surviving fractions using the colony method, the LD/sub 50/, and the growth delay after irradiation of human melanoma cells grown as multicellular spheroids. [/sup 137/Cs]
Journal Article
·
· Radiat. Res.; (United States)
Survival of human melanoma Na 11 cells after ..gamma.. irradiation was measured using three different endpoints: clonogenicity of single cells isolated from spheroids either before or after treatment, probability of cure by sterilization of 50% of the spheroids, and growth delay. No significant difference in radiosensitivity was found between single cells and cells from spheroids. A close relationship was found between the cell yield and the theoretical number of cells per spheroid. Good agreement was found between the LD/sub 50/ survival curve and that from monodispersed cells. When growth delay was plotted against survival a similar conclusion was reached, but with somewhat less precision, indicating that other factors must contribute to regrowth. The multicellular spheroid culture, which permits multiendpoint evaluation of survival, should provide a valid assay of human tumor cell sensitivity to radiation and chemotherapeutic treatments.
- Research Organization:
- Institut de Radiobiologie Clinique, Villejuif France
- OSTI ID:
- 6565266
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 85:2; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL KILLING
CESIUM 137
CESIUM ISOTOPES
DISEASES
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GROWTH
HISTOLOGY
INHIBITION
IONIZING RADIATIONS
ISOTOPES
MAMMALS
MAN
MELANOMAS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
SPHEROIDS
SURVIVAL CURVES
TUMOR CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL KILLING
CESIUM 137
CESIUM ISOTOPES
DISEASES
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
GROWTH
HISTOLOGY
INHIBITION
IONIZING RADIATIONS
ISOTOPES
MAMMALS
MAN
MELANOMAS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
SPHEROIDS
SURVIVAL CURVES
TUMOR CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES