Radiosensitivity of human melanoma spheroids influenced by growth rate

Journal Article · Thu Nov 01 04:00:00 EST 1990 · International Journal of Radiation Oncology, Biology and Physics; (USA)

DOI:https://doi.org/10.1016/0360-3016(90)90227-B· OSTI ID:6168099

Schwachoefer, J H.C.; Crooijmans, R P; Hoogenhout, J; Jerusalem, C R; Kal, H B; Theeuwes, A G ^[1]

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1. University Hospital, Nijmegen (Netherlands)

The radiosensitivity of human melanoma cell line BRO was investigated using the multicellular tumor spheroid system. By adding different concentrations of bovine serum to the tissue culture medium, two different growth rates could be obtained. Spheroids (200-250 microns) were irradiated with graded single doses of X rays (2-8 Gy). The radiation response was quantified using specific growth delay, clonogenic cell survival, and spheroid cure. All three assays showed a growth rate dependent radiation response. At both growth rates the spheroid growth fraction and critical cell number were of comparable magnitude. There was a strong correlation between the radiation response of spheroid regenerating units and clonogenic cells from dispersed spheroids. Cell survival curves indicated a decreased ability to accumulate sublethal damage in fast growing multicellular tumor spheroids. From this study it appears that the intrinsic radiosensitivity of human melanoma cell line BRO cells in multicellular spheroids is modulated by intratumoral conditions.

• OSTI ID: 6168099
• Journal Information: International Journal of Radiation Oncology, Biology and Physics; (USA), Journal Name: International Journal of Radiation Oncology, Biology and Physics; (USA) Vol. 19:5; ISSN IOBPD; ISSN 0360-3016
• Country of Publication: United States
• Language: English

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ANIMAL CELLS
ANIMALS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
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SURVIVAL TIME
TUMOR CELLS
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X RADIATION