Genetics in methylotrophic bacteria: Appendix. Final report
This research has focused primarily on promoters in Methylobacterium extorquens AM1 and in methanotrophic bacteria. In Methylobacterium extorquens work continued on the moxF promoter. The author constructed chromosomal lacZ fusions of this promoter to avoid the regulation problems of plasmid-borne fragments and has shown that this is regulated normally in the chromosome. She has constructed lacZ fusions to some of the mox genes involved in the synthesis of the cofactor, PQQ, in order to carry out similar analysis of transcription of PQQ genes. The author has continued to isolate mox genes in methanotrophs for the purpose of studying their promoters and transcriptional regulation.
- Research Organization:
- California Inst. of Tech., Pasadena, CA (United States)
- Sponsoring Organization:
- USDOE Office of Energy Research, Washington, DC (United States)
- DOE Contract Number:
- FG03-87ER13753
- OSTI ID:
- 656506
- Report Number(s):
- DOE/ER/13753--T7-App.; ON: DE98007390; BR: KC0600000
- Country of Publication:
- United States
- Language:
- English
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