Genetics in methylotrophic bacteria: Appendix. Final report

Technical Report · Tue Sep 01 00:00:00 EDT 1998

DOI:https://doi.org/10.2172/656506· OSTI ID:656506

Lidstrom, M E

This research has focused primarily on promoters in Methylobacterium extorquens AM1 and in methanotrophic bacteria. In Methylobacterium extorquens work continued on the moxF promoter. The author constructed chromosomal lacZ fusions of this promoter to avoid the regulation problems of plasmid-borne fragments and has shown that this is regulated normally in the chromosome. She has constructed lacZ fusions to some of the mox genes involved in the synthesis of the cofactor, PQQ, in order to carry out similar analysis of transcription of PQQ genes. The author has continued to isolate mox genes in methanotrophs for the purpose of studying their promoters and transcriptional regulation.

• Research Organization: California Institute of Technology (CalTech), Pasadena, CA (United States)
• Sponsoring Organization: USDOE Office of Energy Research, Washington, DC (United States)
• DOE Contract Number: FG03-87ER13753
• OSTI ID: 656506
• Report Number(s): DOE/ER/13753-T7-App.; ON: DE98007390; BR: KC0600000; TRN: AHC29817%%61
• Resource Relation: Other Information: PBD: [1998]
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
PROGRESS REPORT
GENETICS
GENE REGULATION
METHANOTROPHIC BACTERIA
TRANSCRIPTION
CHROMOSOMES
GENES
EXPERIMENTAL DATA