Phagocytosis of particulate nickel compounds by rat peritoneal macrophages in vitro

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/3.3.321· OSTI ID:6563065

Kuehn, K; Fraser, C B; Sunderman, Jr, F W

Phagocytic indices of 17 nickel compounds were measured in vitro in monolayer cultures of rat peritoneal macrophages. The macrophages were exposed for 1 h at 37/sup 0/C to particles (1.5 ..mu..m median diameter) of the nickel compounds, at concentrations of 10 ..mu..g/ml of medium (2 ..mu..g/cm/sup 2/ of monolayer). Phagocytic indices (i.e., the percentages of macrophages with one or more engulfed particles) ranged from 69% (NiO) to 3% (amorphous NiS). In order of decreasing phagocytic indices, the 17 nickel compounds were ranked as follows: NiO > Ni/sub 4/FeS/sub 4/ > NiTiO/sub 3/ > NiSe > ..cap alpha..Ni/sub 3/S/sub 2/ > Ni > Ni/sub 5/As/sub 2/ > NiS/sub 2/ > NiFe alloy > NiSb > Ni/sub 11/As/sub 8/ > Ni/sub 3/Se/sub 2/ > ..beta..NiS > NiTe > NiAs > NiAsS > amorphous NiS. Rank-correlation (P < 0.03) was observed between the phagocytic indices of the nickel compounds and their dissolution half-times in rat serum. Nickel subsulfide, ..cap alpha..Ni/sub 3/S/sub 2/, was a notable exception to the general concordance between phagocytic indices and dissolution half-times: ..cap alpha..Ni/sub 3/S/sub 2/ was avidly phagocytized by macrophages, yet it had one of the shortest dissolution half-times. Preliminary results of carcinogenesis tests of 14 of the nickel compounds do not indicate significant rank-correlation between the phagocytic indices of the nickel compounds and the sarcoma incidences at 1 yr after i.m. administration of the compounds to rats.

• Research Organization: Univ. of Connecticut School of Medicine, Farmington
• OSTI ID: 6563065
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 3:3; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism &amp; Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL FUNCTIONS
CARCINOGENESIS
CONNECTIVE TISSUE CELLS
DISEASES
FUNCTIONS
IN VITRO
MACROPHAGES
MAMMALS
MEMBRANES
NEOPLASMS
NICKEL COMPOUNDS
PATHOGENESIS
PERITONEUM
PHAGOCYTES
PHAGOCYTOSIS
PHYSIOLOGY
RATS
RODENTS
SARCOMAS
SEROUS MEMBRANES
SOMATIC CELLS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES