Properties of cellulase immobilized on agarose gel with spacer

Journal Article · Sun Nov 30 23:00:00 EST 1986 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260281215· OSTI ID:6561637

Chim-anage, P; Kashiwagi, Y; Magae, Y; Ohta, T; Sasaki, T

Cellulase produced by fungus Trichoderma viride was immobilized on agarose beads (Sepharose 4B) activated by cyanogen bromide and also on activated agarose beads that contained spacer arm (activated Ch-Sepharose 4B and Affi-Gel 15). The CMCase activity retained by immobilized cellulase on activated Sepharose containing the spacer tended to be higher than that immobilized without spacer, although the extent of protein immobilization was lower. Also, the higher substrate specificity for cellulase immobilized on beads with spacer was obtained for cellobiose, acid-swollen cellulose, or cellulose powder. The hydrolysis product from their substrates was mainly glucose. 10 references.

• Research Organization: National Food Research Institute, Ibaraki, Japan
• OSTI ID: 6561637
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 28:12; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AGAR
ALDEHYDES
CARBOHYDRATES
CELLOBIOSE
CELLULASE
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
COLLOIDS
DECOMPOSITION
DISACCHARIDES
DISPERSIONS
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYMES
FUNGI
GELS
GLUCOSE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PH VALUE
PLANTS
POLYSACCHARIDES
POWDERS
PRODUCTION
PROTEINS
SACCHARIDES
SOLVOLYSIS
SUBSTRATES
TRICHODERMA
TRICHODERMA VIRIDE