Properties of cellulase immobilized on agarose gel with spacer
Abstract
Cellulase produced by fungus Trichoderma viride was immobilized on agarose beads (Sepharose 4B) activated by cyanogen bromide and also on activated agarose beads that contained spacer arm (activated Ch-Sepharose 4B and Affi-Gel 15). The CMCase activity retained by immobilized cellulase on activated Sepharose containing the spacer tended to be higher than that immobilized without spacer, although the extent of protein immobilization was lower. Also, the higher substrate specificity for cellulase immobilized on beads with spacer was obtained for cellobiose, acid-swollen cellulose, or cellulose powder. The hydrolysis product from their substrates was mainly glucose. 10 references.
- Authors:
- Publication Date:
- Research Org.:
- National Food Research Institute, Ibaraki, Japan
- OSTI Identifier:
- 6561637
- Resource Type:
- Journal Article
- Journal Name:
- Biotechnol. Bioeng.; (United States)
- Additional Journal Information:
- Journal Volume: 28:12
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; CELLULASE; ENZYME ACTIVITY; IMMOBILIZED ENZYMES; AGAR; CELLOBIOSE; CHEMICAL ACTIVATION; ENZYMATIC HYDROLYSIS; GELS; GLUCOSE; PH VALUE; POWDERS; PRODUCTION; PROTEINS; SUBSTRATES; TRICHODERMA VIRIDE; ALDEHYDES; CARBOHYDRATES; CHEMICAL REACTIONS; COLLOIDS; DECOMPOSITION; DISACCHARIDES; DISPERSIONS; ENZYMES; FUNGI; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; HYDROLYSIS; LYSIS; MONOSACCHARIDES; O-GLYCOSYL HYDROLASES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; TRICHODERMA; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 550700 - Microbiology; 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
Citation Formats
Chim-anage, P, Kashiwagi, Y, Magae, Y, Ohta, T, and Sasaki, T. Properties of cellulase immobilized on agarose gel with spacer. United States: N. p., 1986.
Web. doi:10.1002/bit.260281215.
Chim-anage, P, Kashiwagi, Y, Magae, Y, Ohta, T, & Sasaki, T. Properties of cellulase immobilized on agarose gel with spacer. United States. https://doi.org/10.1002/bit.260281215
Chim-anage, P, Kashiwagi, Y, Magae, Y, Ohta, T, and Sasaki, T. 1986.
"Properties of cellulase immobilized on agarose gel with spacer". United States. https://doi.org/10.1002/bit.260281215.
@article{osti_6561637,
title = {Properties of cellulase immobilized on agarose gel with spacer},
author = {Chim-anage, P and Kashiwagi, Y and Magae, Y and Ohta, T and Sasaki, T},
abstractNote = {Cellulase produced by fungus Trichoderma viride was immobilized on agarose beads (Sepharose 4B) activated by cyanogen bromide and also on activated agarose beads that contained spacer arm (activated Ch-Sepharose 4B and Affi-Gel 15). The CMCase activity retained by immobilized cellulase on activated Sepharose containing the spacer tended to be higher than that immobilized without spacer, although the extent of protein immobilization was lower. Also, the higher substrate specificity for cellulase immobilized on beads with spacer was obtained for cellobiose, acid-swollen cellulose, or cellulose powder. The hydrolysis product from their substrates was mainly glucose. 10 references.},
doi = {10.1002/bit.260281215},
url = {https://www.osti.gov/biblio/6561637},
journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 28:12,
place = {United States},
year = {Mon Dec 01 00:00:00 EST 1986},
month = {Mon Dec 01 00:00:00 EST 1986}
}
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