Use of mammalian cells to investigate the genetic consequences of DNA damage induced by ionizing radiation

Conference · Wed Nov 30 23:00:00 EST 1988

OSTI ID:6558073

Morgan, T L; Fleck, E W; Poston, K A; Denovan, B A; Newman, C N; Rossiter, B J.F.; Miller, J H

The present work was conducted to determine the effects of cellular repair processes on the spectrum of mutations induced in CHO-K1 cells. We investigated the effects of dose, delayed replating and dose fractionation on the yield, type and position of mutations induced at the HPRT locus by 250 kVp X rays. Dose was the only parameter which showed a statistically significant effect on the mutation spectrum. At 2 Gy the fraction of mutants containing complete deletions of the gene was less than that observed with a dose of 4 Gy. 22 refs., 1 fig., 2 tabs.

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830; AM06-70RL02225
• OSTI ID: 6558073
• Report Number(s): PNL-SA-15899; CONF-8810209-8; ON: DE89005157
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CELL CULTURES
CLONING
DAMAGE
DNA
DNA HYBRIDIZATION
DNA REPAIR
DNA-CLONING
DOSE-RESPONSE RELATIONSHIPS
EVALUATION
HYBRIDIZATION
IONIZING RADIATIONS
MESSENGER-RNA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLASMIDS
RADIATIONS
RECOVERY
REPAIR
RNA
STRUCTURE-ACTIVITY RELATIONSHIPS