Molecular analysis of gamma-ray-induced mutations at the hprt locus in primary human skin fibroblasts by multiplex polymerase chain reaction
- Los Alamos National Lab., NM (United States)
A total of 153 hprt mutants (23 spontaneous, 130 {gamma}-ray-induced) of primary human skin fibroblasts were isolated and genetic alterations at the locus were studied by multiplex polymerase chain reaction (PCR). The analyses showed that 51% (66/130) of {gamma}-ray-induced genetic changes were large deletions, whereas the majority of spontaneous mutants (21/23) exhibited point mutations. The spectrum of large genetic alterations appeared to be dependent on dose in {gamma}-ray-induced (1-4 Gy) mutations; mutants with complete loss of the hprt locus comprised 21 (3/14) or 39% (15/38) of clones isolated after irradiation with 1 or 4 Gy, respectively. The frequency of partial deletions was found to be higher in the mutants isolated from clones irradiated with 2 Gy (38%) than from those irradiated with 4 Gy (8%). Mapping of all intragenic depletion breakpoints exhibited a nonrandom distribution of breakpoints toward the 3{prime} end of the hprt gene. 46 refs., 4 figs., 2 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 62044
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Journal Issue: 1 Vol. 141; ISSN RAREAE; ISSN 0033-7587
- Country of Publication:
- United States
- Language:
- English
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