Determination of 2,3,7,8-TCDD Toxic Equivalent Factors (TEFS): Support for the use of the in vitro AHH induction assay
The in vitro induction of the cytochrome P1-450-dependent monooxygenases, aryl hydrocarbon hydroxylase (AHH) or ethoxyresorufin O-deethylase (EROD) by 2,3,7,8-TCDD and related toxic halogenated aryl hydrocarbons in rat hepatoma H-4-II E cells has been developed as a short term quantitative bioassay for these toxic chemicals. There was a linear correlation between the -log EC50 (in vitro) AHH induction vs the -log ED50 (in vivo) for body weight loss, thymic atrophy, hepatic AHH and EROD induction in the rat for several polychlorinated biphenyl, dibenzo-p-dioxin and dibenzofuran congeners and mixtures. These data clearly support the utility of the in vitro AHH induction assay as a short term test system for quantitating the toxic or 2,3,7,8-TCDD equivalent in an extract containing toxic halogenated aromatics. The bioassay method is rapid, relatively accurate and much more cost effective than conventional analytical methods such as gas chromatography-mass spectrometry from which it is difficult to determine the levels of 2,3,7,8-TCDD equivalents in specific analytes.
- Research Organization:
- Texas A and M Univ., College Station, TX (USA)
- OSTI ID:
- 6552201
- Report Number(s):
- PB-90-264987/XAB; CNN: EPA-810995
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOFURANS
BIOASSAY
CHLORINATED AROMATIC HYDROCARBONS
DIOXIN
DISEASES
DISPERSIONS
ENZYME INDUCTION
ENZYMES
FURANS
GENE REGULATION
HALOGENATED AROMATIC HYDROCARBONS
HEPATOMAS
HETEROCYCLIC COMPOUNDS
HYDROXYLASES
IN VITRO
MAMMALS
MIXED-FUNCTION OXIDASES
MIXTURES
NEOPLASMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
RATS
RODENTS
TOXICITY
TUMOR CELLS
VERTEBRATES