Immunoreactive helix-destabilizing protein localized in transcriptionally active regions of Drosophila polytene chromosomes
A highly purified helix-destabilizing protein (HDP) obtained from rat liver has been used to elicit specific, high-titer anti-HDP sera in rabbits. These antisera show immunological crossreaction with single-stranded DNA binding proteins from several very diverse eukaryotic sources, including Drosophila embryos. The use of such antisera in the labeling of Drosophila salivary gland chromosomes buy indirect immunofluorescence shows concentrations of immunoreactive HDP in many regions, but especially in chromosome puffs. There is a striking localization of HDP in heat shock puffs known to be sites of new transcription. The pattern of HDP distribution seems to implicate a transcriptional role, with some specificities independent of puffing itself.
- Research Organization:
- Univ. of Georgia, Athens
- OSTI ID:
- 6551923
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 77:11; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BINDING ENERGY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CHROMOSOMES
CONFIGURATION
DIGESTIVE SYSTEM
DNA
DNA REPLICATION
ENERGY
ENZYME ACTIVITY
FLUORESCENCE
GENETIC EFFECTS
GLANDS
HELICAL CONFIGURATION
IMMUNE SERUMS
INOCULATION
KINETICS
LIVER
LUMINESCENCE
MAMMALS
METABOLISM
MOLECULAR STRUCTURE
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
QUANTITY RATIO
RATS
REACTION KINETICS
RODENTS
SALIVARY GLANDS
SENSITIVITY
TIME DEPENDENCE
TRANSCRIPTION
VERTEBRATES