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Regions of the polytene chromosomes of Drosophila virilis carrying multiple dispersed p Dv 111 DNA sequences

Journal Article · · Sov. Genet. (Engl. Transl.); (United States)
OSTI ID:5785043
;
The cloned sequences of p Dv 111 DNA hybridized in situ with more than 170 regions of Drosophila virilis salivary gland chromosomes. Comparative autoradiography of in situ hybridization and the nature of pulse /sup 3/H-thymidine and /sup 3/H-deoxycytidine incorporation into the polytene chromosomes of D. virilis at the puparium formation stage showed that the hybridization sites of p Dv 111 are distributed not only in the heterochromatic regions but also in the euchromatic regions of the chromosomes that are not late replicating. Two distinct bands of hybridization of p Dv 111 /sup 3/H-DNA were observed in the region of the heat shock puff 20CD. The regions of the distal end of chromosome 2, in which breaks appeared during radiation-induced chromosomal rearrangements, hybridized with the p Dv 111 DNA.
Research Organization:
Institute of Molecular Biology and Genetics, Kiev, USSR
OSTI ID:
5785043
Journal Information:
Sov. Genet. (Engl. Transl.); (United States), Journal Name: Sov. Genet. (Engl. Transl.); (United States) Vol. 22:3; ISSN SOGEB
Country of Publication:
United States
Language:
English

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Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTHROPODS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL SHOCK
BIOLOGY
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DIPTERA
DNA
DNA REPLICATION
DNA SEQUENCING
DROSOPHILA
FLIES
FRUIT FLIES
GENES
GENETICS
HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
INSECTS
INVERTEBRATES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MUTATIONS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PUPAE
PYRIMIDINES
RADIONUCLIDE KINETICS
RIBOSIDES
STRAND BREAKS
STRUCTURAL CHEMICAL ANALYSIS
THERMAL SHOCK
THYMIDINE
TRACER TECHNIQUES
TRANSCRIPTION
TRITIUM COMPOUNDS
UPTAKE