Immunolocalization of neuroblastoma using radiolabeled monoclonal antibody UJ13A

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · J. Pediatr.; (United States)

DOI:https://doi.org/10.1016/S0022-3476(84)80122-5· OSTI ID:6551578

Goldman, A; Vivian, G; Gordon, I; Pritchard, J; Kemshead, J

The monoclonal antibody UJ13A, raised after immunization of mice with human fetal brain, recognized an antigen expressed on human neuroblastoma cell lines and fresh tumors. Antibody was purified and radiolabeled with iodine isotopes using chloramine-T. In preclinical studies, 125I-labeled UJ13A was injected intravenously into nude mice bearing xenografts of human neuroblastoma. Radiolabeled UJ13A uptake by the tumors was four to 23 times greater than that by blood. In control animals, injected with a similar quantity of a monoclonal antibody known not to bind to neuroblastoma cells in vitro (FD44), there was no selective tumor uptake. Nine patients with histologically confirmed neuroblastoma each received 100 to 300 micrograms UJ13A radiolabeled with 1 to 2.8 mCi 123I or 131I. Sixteen positive sites were visible on gamma scans 1 to 7 days after injection: 15 were primary or secondary tumor sites, and one was a false positive; there were two false negatives. In two of the 15 positive sites, tumor had not been demonstrated by other imaging techniques; these were later confirmed as areas of malignant infiltration. No toxicity was encountered.

• Research Organization: Department of Hematology, Hospital for Sick Children, London, England
• OSTI ID: 6551578
• Journal Information: J. Pediatr.; (United States), Journal Name: J. Pediatr.; (United States) Vol. 105:2; ISSN JOPDA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
CARCINOMAS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NERVOUS SYSTEM DISEASES
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPE SCANNING
RADIOISOTOPES
RODENTS
USES
VERTEBRATES