Degradation of /sup 14/C-labeled lignins and /sup 14/C-labeled aromatic acids by fusarium solani

Journal Article · Fri Aug 01 04:00:00 EDT 1980 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6551412

Norris, D M

Abilities of isolate AF-W1 of Fusarium solani to degrade the side chain and the ring structure of synthetic dehydrogenative polymerizates, aromatic acids, or lignin in sound wood were investigated under several conditions of growth substrate or basal medium and pH. Significant transformations of lignins occurred in 50 days in both unextracted and extracted sound wood substrances with 3% malt as the growth substrate and the pH buffered initially at 4.0 with 2,2-dimethylsuccinate. Degradation of lignin in such woods also occurred under unbuffered pH conditions when a basal medium of either 3% malt or powdered cellulose in deionized water was present. Decomposition of the lignin in these woods did not occur in cultures where D-glucose was present as a growth substrate. F. solani significantly transformed, as measured as evolved /sup 14/CO/sub 2/, both synthetic side chain (beta, gamma)-/sup 14/C- and U-ring-/sup 14/C-labeled lignins in 30 days under liquid culture conditions of only distilled deionized water and no pH adjustment. Degradation of dehydrogenative polymerizates by F. solani was reduced drastically when D2 was the liquid medium. AF-W1 also cleaved the alpha-/sup 14/C from p- hydroxybenzoic acid and evolved /sup 14/CO/sub 2/ from the substrace, (3-/sup 14/C) cinnamic acid. Thus, the fungus cleaved side chain carbon from substrate that originally lacked hydroxyl substitution on the aromatic nucleus. Surprisingly, small amounts of /sup 14/C cleaved from aromatic acids by F. solani were incorporated into cell mass. Initial buffering of the culture medium to pH 4.0 or 5.0 with 0.1 M2,2-dimethylsuccinate significantly increased F. solani degradation of all lignins or aromatic acids. Results indicated that AF-W1 used lignin as a sole carbon source.

• Research Organization: Russell Laboratories, Univ of Wisconsin, Madison, Wisconsin 53706
• OSTI ID: 6551412
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 40:2; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIODEGRADATION
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECOMPOSITION
FUNGI
FUSARIUM
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGNIN
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PLANTS
POLYSACCHARIDES
SACCHARIDES
TRACER TECHNIQUES