Enhanced radiosensitivity of cultured fibroblasts from ataxia telangiectasia heterozygotes manifested by defective colony-forming ability and reduced DNA repair replication after hypoxic. gamma. -irradiation. [/sup 60/Co]

Journal Article · Sat Sep 01 04:00:00 EDT 1979 · Cancer Res.; (United States)

OSTI ID:6551173

Paterson, M C; Anderson, A K; Smith, B P; Smith, P J

We have measured the sensitivity to ..gamma..-ray inactivation of diploid skin fibroblasts cultured from 10 persons in four families with ataxia telangiectasia (AT). Persons heterozygous for AT, including parents of afflicted patients, are not as yet detectable by any specific clinical or laboratory marker but are believed to constitute a substantial portion of the middle-aged cancer population. In one AT family, fibroblast strains from both parents exhibited a colony-forming ability after hypoxic irradiation which was intermediate between that displayed by five control strains from normal children and that from the affected child. In the remaining three families, cultures from only one parent were available; one parental strain displayed an intermediate survival capacity as above, whereas the other two responded normally. The homozygous recessive strains from the five afflicted children in the four families were all equally hypersensitive to hypoxic ..gamma..-ray inactivation. The three presumed AT heterozygous strains that displayed intermediate rayiosensitivity also carried out ..gamma..-rad-induced DNA repair replication to an extent intermediate between those in normals and AT homozygotes. These findings suggest that a numerically significant, cancer-prone subpopulation of humans carrying one normal and one abnormal AT gene may also be moderately sensitive to lethal effects of hypoxic ..gamma..-rays due to a defect in the enzymatic repair of DNA.

• Research Organization: Chalk River Nuclear Labs., Ontario
• OSTI ID: 6551173
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 39; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANOXIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL KILLING
COBALT 60
COBALT ISOTOPES
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DISEASES
DNA REPLICATION
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GAMMA RADIATION
HYBRIDIZATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACID REPLICATION
ODD-ODD NUCLEI
ORGANS
PATIENTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
SKIN
SKIN DISEASES
SOMATIC CELLS
SURVIVAL CURVES
TELANGIECTASIS
VASCULAR DISEASES
YEARS LIVING RADIOISOTOPES