Evidence for loss of tritium from 3 beta-tritiated deoxycholic acid during enterohepatic cycling in man

Journal Article · Wed Jun 01 04:00:00 EDT 1988 · Lipids; (United States)

OSTI ID:6544475

Marcus, S N; Heaton, K W

A double-label single-intubation isotope technique was assessed for studying deoxycholic acid metabolism by simultaneous IV administration of (3 beta-/sup 3/H)deoxycholic acid and (24-/sup 14/C)deoxycholic acid to five healthy subjects. The /sup 3/H//sup 14/C ratio in duodenal bile fell progressively in all subjects, the mean reduction on day four being 48.8%. When the same labeled bile acids were administered to three ileostomy subjects, again the ratio fell with a mean reduction of 44.4% on day four. This fall more likely was due to hepatic exchange of tritium for hydrogen, rather than to colonic bacterial oxidation. (3 beta-/sup 3/H)Deoxycholic acid should not be used to study the kinetics and metabolism of deoxycholic acid.

• Research Organization: Univ. of California, San Diego, La Jolla (USA)
• OSTI ID: 6544475
• Journal Information: Lipids; (United States), Journal Name: Lipids; (United States) Vol. 23:6; ISSN LPDSA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BILE ACIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHOLIC ACID
DOUBLE LABELLING
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPE RATIO
LABELLED COMPOUNDS
LABELLING
METABOLISM
ORGANIC ACIDS
ORGANIC COMPOUNDS
STEROIDS
STEROLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS