Oxidoreduction of different hydroxyl groups in bile acids during their enterohepatic circulation in man
The extent of oxidoreduction of the 3 alpha-, 7 alpha- and 12 alpha-hydroxyl groups in bile acids during the enterohepatic circulation in man was studied with the use of (3 beta-/sup 3/H)-labeled deoxycholic acid and cholic acid, (7 beta-/sup 3/H)-labeled cholic acid, and (12 beta-/sup 3/H)-labeled deoxycholic acid and cholic acid. Each (/sup 3/H)-labeled bile acid was given per os to healthy volunteers, together with the corresponding (24-/sup 14/C)-labeled bile acid. The rate of oxidoreduction was calculated from the decrease in the ratio between /sup 3/H and /sup 14/C in the respective bile acid isolated from duodenal contents collected at different time intervals after administration of the labeled bile acids. The mean fractional conversion rate was found to be 0.29 day-1 for the 3 alpha-hydroxyl group in deoxycholic acid (n = 2), 0.18 day-1 for the 12 alpha-hydroxyl group in deoxycholic acid (n = 6), 0.09 day-1 for the 3 alpha-hydroxyl group in cholic acid (n = 3), 0.05 day-1 for the 7 alpha-hydroxyl group in cholic acid (n = 2), and 0.03 day-1 for the 12 alpha-hydroxyl group in cholic acid (n = 2). The extent of oxidoreduction of the 12 alpha-hydroxyl group in (12 beta-/sup 3/H)-labeled deoxycholic acid given to two patients operated with subtotal colectomy and ileostomy was markedly reduced (less than 20% of normal).
- Research Organization:
- Huddinge Univ. Hospital, Sweden
- OSTI ID:
- 5592275
- Journal Information:
- J. Lipid Res.; (United States), Journal Name: J. Lipid Res.; (United States) Vol. 2; ISSN JLPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BILE ACIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHOLIC ACID
DIGESTIVE SYSTEM DISEASES
DISEASES
HYDROXY COMPOUNDS
HYDROXYLATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
MAMMALS
MAN
MEDICINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
PRIMATES
REDOX REACTIONS
STEROIDS
STEROLS
SURGERY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES