Effect of antioxidants of aflatoxigenic molds
Growth and aflatoxin production by Aspergillus flavus and A. parasiticus were measured in synthetic media and chicken feed treated with butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), ethoxyquin (EXQ), propyl gallate (PG) or t-butylhydroquinone (TBHQ) at 50-250 ..mu..g/g and 50-1000 ..mu..g/g, respectively. BHA at 50 ..mu..g/g in broth or agar inhibited growth and alfatoxin production by both molds. In poultry feeds, 1000 ..mu..g/g BHA was required to depress CO/sub 2/ production at day 5 of incubation, while 200 ..mu..g/g decreased aflatoxin B/sub 1/ 23.8%. BHT, EXQ, and PG had slight effects on mold growth while TBHQ enhanced growth on broth but reduced it on agar. Afaltoxin B/sub 1/ production was stimulated by EXQ, although G/sub 1/ production declines. Measurement of cellular constituents released from mycelia over 5 hours indicated that BHA doubled the release of UV-absorbing materials and increase by threefold the release of previously incorporated radiolabeled compounds. Furthermore, BHA inhibited the uptake of /sup 14/C-amino acids 8 and 44%, /sup 3/H-uridine 65 and 91%, and /sup 3/H-methyl thymidine 67 and 71%, for A. flavus and A. parasiticus, respectively, compared to untreated controls.
- Research Organization:
- Maryland Univ., College Park (USA)
- OSTI ID:
- 6544010
- Resource Relation:
- Other Information: Thesis (Ph. D)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANISOLE
TOXICITY
ANTIOXIDANTS
ASPERGILLUS
GROWTH
GALLIC ACID
QUINONES
TOLUENE
AFLATOXIN
AMINO ACIDS
ANIMAL FEEDS
CARBON 14 COMPOUNDS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URIDINE
ALKYLATED AROMATICS
ANTIGENS
AROMATICS
AZINES
CARBOXYLIC ACIDS
ETHERS
FOOD
FUNGI
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY ACIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MATERIALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PLANTS
PYRIMIDINES
RIBOSIDES
TOXIC MATERIALS
TOXINS
URACILS
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques