On-line mass spectrometric detection for capillary zone electrophoresis
The authors have developed a viable alternative to capillary zone electrophoresis (CZE) detection based on mass spectrometric interfacing. A capillary zone electrophoresis-mass spectrometry (CZE-MS) interface obviously requires a substantial departure from the conventional CZE arrangement; it is clear that the interface design and ionization method are crucial to success. The liquid flow rate in CZE (approx.1 ..mu..L/min) is highly compatible with conventional mass spectrometers even if the total column effluent was introduced directly. The direct liquid introduction interfaces developed for LC-MS suffer from orifice plugging at low flow rates and the thermal degradation of high mass-low volatility components. Thermospray ionization, though attractive, has not been shown to be effective for liquid flow rates below a few tenths of a mL/min. Therefore, the authors evaluation of the requirements for a mass spectrometer interface suggested an approach that incorporates the electrospray ionization technique developed by Dole et al. and the more recent work reported by Fenn and co-workers. In this communication, the authors report the successful development of CZE-MS instrumentation for the separation and analysis of ionic species in aqueous solutions.
- Research Organization:
- Pacific Northwest Lab., Richland, WA
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 6543480
- Journal Information:
- Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 59:8; ISSN ANCHA
- Country of Publication:
- United States
- Language:
- English
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An improved interface for capillary zone electrophoresis-mass spectrometry
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Related Subjects
400102 -- Chemical & Spectral Procedures
400105* -- Separation Procedures
AMMONIUM COMPOUNDS
AQUEOUS SOLUTIONS
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
CHEMICAL ANALYSIS
DATA
DISPERSIONS
ELECTROPHORESIS
EXPERIMENTAL DATA
HYDROGEN COMPOUNDS
INFORMATION
MASS SPECTROSCOPY
MIXTURES
NUMERICAL DATA
ORGANS
OXYGEN COMPOUNDS
QUALITATIVE CHEMICAL ANALYSIS
QUANTITATIVE CHEMICAL ANALYSIS
SALTS
SEPARATION PROCESSES
SOLUTIONS
SPECTROSCOPY
WATER