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Specific radioimmunoassay for estrone sulfate in plasma and urine without hydrolysis. [Tritium tracer technique]

Journal Article · · J. Clin. Endocrinol. Metab.; (United States)
; ; ;
Estrone sulfate, quantitatively the most important estrogen in plasma, has previously been determined only after hydrolysis and chromatography. An antiserum raised against estrone glucosiduronate-bovine thyroglobulin was found to be suitable for the specific RIA of estrone sulfate both in plasma and urine. Plasma levels were measured after solvent extraction without hydrolysis or chromatography. The mean (+-SE) was 972 +- 79 pg/ml (range, 537 to 1581) in 15 women in the follicular phase, 1806 +- 160 pg/ml (range, 814 to 3358) in 15 women in the luteal phase, and 922 +- 62 pg/ml (range, 461 to 1238) in 13 men. The urinary excretion of estrone sulfate, measured after simple chromatographic separation, ranged from 0.8 to 7.9 ..mu..g/24 h in men and 5.1 to 18.7 ..mu..g/24 h in nonpregnant women. This was generally one-seventh to one-half the simultaneous estrone glucosiduronate excretion rate. An approximate mean renal clearance of estrone sulfate calculated from the above values was 2.7 ml/min. The low clearance rate is taken to reflect extensive binding of estrone sulfate by plasma proteins. The splanchnic extraction of estrone sulfate measured in 6 patients undergoing hepatic vein catheterization for diagnostic purposes was 29.8 +- 11.1%, indicating net uptake of this compound by the splanchnic area.
Research Organization:
Emory Univ. School of Medicine, Atlanta
OSTI ID:
6542653
Journal Information:
J. Clin. Endocrinol. Metab.; (United States), Journal Name: J. Clin. Endocrinol. Metab.; (United States) Vol. 47:5; ISSN JCEMA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BLOOD PLASMA
BODY FLUIDS
CLEARANCE
ESTRANES
ESTROGENS
ESTRONE
EXCRETION
FEMALES
HORMONES
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
MAN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RENAL CLEARANCE
REPRODUCTION
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
TRITIUM
URINE
VERTEBRATES
WASTES
WOMEN
YEARS LIVING RADIOISOTOPES