Regulation of (/sup 3/H)GABA release from strips of guinea pig urinary bladder

Journal Article · Thu Dec 01 04:00:00 EST 1988 · Am. J. Physiol.; (United States)

OSTI ID:6540849

Shirakawa, J; Taniyama, K; Iwai, S; Tanaka, C

The presence of receptors that regulate the release of gamma-aminobutyric acid (GABA) was studied in strips of the guinea pig urinary bladder. GABA (10(-8)-10(-5) M) and muscimol (10(-8)-10(-5) M), but not baclofen (10(-5) M), reduced the Ca2+-dependent, tetrodotoxin-resistant release of (/sup 3/H)GABA evoked by high K+ from the urinary bladder strips preloaded with (/sup 3/H)GABA. The inhibitory effect of muscimol was antagonized by bicuculline and potentiated by diazepam, clonazepam, and pentobarbital sodium. The potentiating effect of clonazepam was antagonized by Ro 15-1788. Acetylcholine (ACh) inhibited the high K+-evoked release of (/sup 3/H)GABA. The inhibitory effect of ACh was antagonized by atropine sulfate and pirenzepine but not by hexamethonium. Norepinephrine (NE) inhibited the evoked release of (/sup 3/H)GABA. The inhibitory effect of NE was mimicked by clonidine, but not by phenylephrine, and was antagonized by yohimbine but not by prazosin. These results provide evidence that the release of GABA from strips of guinea pig urinary bladder is regulated via the bicuculline-sensitive GABAA receptor, M1-muscarinic, and alpha 2-adrenergic receptors.

• Research Organization: Kobe Univ., School of Medicine (Japan)
• OSTI ID: 6540849
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 255; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALKALI METALS
ALKALOIDS
AMINES
AMINO ACIDS
AMINOBUTYRIC ACID
AMMONIUM COMPOUNDS
ANIMALS
ATROPINE
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BLADDER
BODY
CARBOXYLIC ACIDS
DRUGS
ELEMENTS
ESTERS
GUINEA PIGS
IN VITRO
INHIBITION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
METALS
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
POTASSIUM
PROTEINS
QUATERNARY COMPOUNDS
REACTION KINETICS
RECEPTORS
RODENTS
SECRETION
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINARY TRACT
VERTEBRATES