Modulation of acetylcholine release from rat striatal slices by the GABA/benzodiazepine receptor complex
GABA, THIP and muscimol enhance spontaneous and inhibit electrically induced release of tritium labelled compounds from rat striatal slices which have been pre-labelled with /sup 3/H-choline. Baclofen is inactive in this model. Muscimol can inhibit electrically induced release of tritiated material by approximately 75% with half maximal effects at 2 ..mu..M. The response to muscimol can be blocked by the GABA antagonists bicuculline methobromide, picrotoxin, anisatin, R 5135 and CPTBO (cyclopentylbicyclophosphate). Drugs which act on the benzodiazepine receptor (BR) require the presence of muscimol to be effective and they modulate the effects of muscimol in a bidirectional manner. Thus BR agonists enhance and inverse BR agonists attenuate the inhibitory effects of muscimol on electrically induced release. Ro15-1788, a BR antagonist, does not modulate the inhibitory effects of muscimol but antagonizes the actions of clonazepam, a BR agonist, and of DMCM, an inverse BR agonist. These results demonstrate that a GABA/benzodiazepine receptor complex can modulate acetylcholine release from rat striatal slices in vitro. 24 references, 3 figures, 5 table.
- Research Organization:
- Sandoz Ltd., Basle, Switzerland
- OSTI ID:
- 5416281
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 36:5; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALCOHOLS
AMINES
AMINO ACIDS
AMINOBUTYRIC ACID
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMISTRY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM AGENTS
CHEMISTRY
CHOLINE
DRUGS
ESTERS
FUNCTIONAL MODELS
HYDROXY COMPOUNDS
IN VITRO
LABELLED COMPOUNDS
LIPOTROPIC FACTORS
MAMMALS
MUSCLES
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
PSYCHOTROPIC DRUGS
QUATERNARY COMPOUNDS
RATS
RECEPTORS
RODENTS
TRANQUILIZERS
TRITIUM COMPOUNDS
VERTEBRATES