Induction of immune resistance against L1210 lymphatic leukemia in mice after chemoradiotherapy of the leukemia and reconstitution with bone marrow purged from the leukemia with mafosfamide

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Exp. Hematol.; (United States)

OSTI ID:6540637

Skorski, T; Kawalec, M

Lymphatic leukemia L1210-bearing semisyngeneic Balb/c x DBA/2Wf F1 (CD2F1) mice were subjected to chemoradiotherapy (2 x 100 mg/kg of cyclophosphamide i.p. and 1000 cGy of total body irradiation) and reconstitution with 10(7) syngeneic bone marrow cells i.v. The bone marrow obtained from leukemic mice was previously ex vivo purged of the leukemia cells with mafosfamide (ASTA Z7654) and stored in liquid nitrogen. Eight weeks after cytoreductive therapy and bone marrow transplantation we tried to immunize the mice against the lethal dose of the leukemia by i.p. injections of L1210-Maf cells (L1210 cells treated in vitro with mafosfamide for inhibition of their growth). About 75% of such mice were able to reject the subsequent 10(3) L1210 leukemia cell challenge, as compared with 70% of normal immunized mice and 55% of mice reconstituted with bone marrow cells not treated with mafosfamide.

• Research Organization: Medical Centre of Postgraduate Education, Warsaw (Poland)
• OSTI ID: 6540637
• Journal Information: Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 16:9; ISSN EXHMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BONE MARROW CELLS
COMBINED THERAPY
CONNECTIVE TISSUE CELLS
DISEASES
EXTERNAL IRRADIATION
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
IMMUNITY
IRRADIATION
LEUKEMIA
MAMMALS
MICE
NEOPLASMS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
THERAPY
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION