Effects of ethylene glycol monomethyl (EGME) and monoethyl (EGEE) ethers on the immunocompetence of allogeneic and syngeneic mice bearing L1210 mouse leukemia

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · Environ. Health Perspect.; (United States)

DOI:https://doi.org/10.1289/ehp.8457113· OSTI ID:5705903

Houchens, D P; Ovejera, A A; Niemeier, R W

The effect of ethylene glycol monomethyl ether and ethylene glycol monoethyl ether on cell-mediated immunity was evaluated by an allograft rejection assay. Allogeneic B6C3F/sub 1/ (C57BL/6 X C3H) mice were given oral doses of 600, 1200, or 2400 mg/kg/administration of EGEE or 300, 600, 1200 mg/kg/administration of EGME on days -12 through -8 or cyclophosphamide at 180 mg/kg by the IP route on day -1. Untreated controls were given oral doses of water on days -12 through -8 and -5 through -1. On day 0, the mice were challenged with 1 X 10/sup 2/, 3 X 10/sup 3/, and 1 X 10/sup 5/ or 3 X 10/sup 6/ L1210 cells by the IP route. Syngeneic CD2F/sub 1/ (Balb/c X DBA/2) mice were challenged with 1 X 10/sup 5/ L1210 cells on day 0 and were treated on days 1 to 5 and 8 to 12 with the same dosages of EGME and EGEE used for the B6C3F/sub 1/ mice. Water-treated syngeneic mice died with a median survival time (MST) of 8.0 days. There was no effect on the MST of syngeneic mice treated with either EGME or EGEE, indicating no direct antitumor effect of the compounds. All Allogeneic mice receiving either water of Cy and challenged with 3 X 10/sup 6/ tumor cells, died with ascites. However, when mice were treated with EGME or EGEE and challenged with 3 X 10/sup 6/ tumor cells, no more than one animal per group died. This would indicate that there was a prophylactic action of the compounds or that the immune system was stimulated. Blood smears of allogeneic mice were made for differential counts the last day of drug dosing, the day of death where possible, and on survivors at day 43 post-tumor implantation. Differential counts showed evidence of monocytosis, considered to be indicative of monocytic leukemia, in those mice not surviving until the day of sacrifice. 15 references, 6 tables.

• Research Organization: Battelle Memorial Inst., Columbus, OH
• OSTI ID: 5705903
• Journal Information: Environ. Health Perspect.; (United States), Journal Name: Environ. Health Perspect.; (United States) Vol. 57; ISSN EVHPA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AMIDES
ANIMAL CELLS
ANIMALS
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ETHERS
ETHYL ETHER
GLYCOLS
HEMIC DISEASES
HYDROXY COMPOUNDS
IMMUNE REACTIONS
IMMUNITY
LEUKEMIA
MAMMALS
MATERIALS
METHYL ETHER
MICE
NEOPLASMS
ORAL ADMINISTRATION
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
RODENTS
SURVIVAL TIME
TOXICITY
TUMOR CELLS
VERTEBRATES