The impact of energy-related pollutants on chromosome structure: Progress report, May 1, 1988--April 30, 1989
We have examined, at the DNA sequence level, the sequence preferences of binding of six chemicals to linear DNA. The chemicals utilized were the bis-(1,10)-phenanthroline Cu(I) complex ((Phen)/sub 2/Cu/sup +/), (+)-anti-and (/minus/)-anti isomers of benzo(a)pyrene-7,8-diol-9, 10-epoxide (BPDE), acetylacetoxyamino-fluorene (AAAF), 8-azido-ethidium (AEt), and 7-azido-actinomycin D (AAct D). Mapping of binding sites depends on the abilities of these chemicals to either spontaneously cleave DNA, as in the case of (Phen)/sub 2/Cu/sup +/, or to create alkali-labile base adducts, as in the cases of the other chemicals. The techniques utilized are analogous to those of DNA sequencing. Binding sites were mapped in sea urchin and mouse histone genes cloned into pUC plasmids. Mapping has now been performed on 1000--2000 bases for all of the chemicals excepting AAAF. All six chemical exhibit distinct sequence preferences. Preferences are notable at the level of doublets, but in all cases the preferences are strongest at the levels of triplets or quartets. Some evidence has been obtained for preferences that extend beyond quartets. The most preferred sequences are different for all five chemicals examined in detail. Perhaps most interesting are the differences in specifications noted for the anti-(+) and anti-(/minus/) isomers of BPDE.
- Research Organization:
- Florida State Univ., Tallahassee (USA). Inst. of Molecular Biophysics
- DOE Contract Number:
- FG05-87ER60588
- OSTI ID:
- 6540135
- Report Number(s):
- DOE/ER/60588-2; ON: DE89005269
- Country of Publication:
- United States
- Language:
- English
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The impact of energy related pollutants on chromosome structure
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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLAMINOFLUORENES
ACTINOMYCIN
ADDUCTS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AQUATIC ORGANISMS
AROMATICS
AZAARENES
BENZOPYRENE
CODONS
CONDENSED AROMATICS
DNA ADDUCTS
DNA SEQUENCING
DOCUMENT TYPES
DRUGS
ECHINODERMS
HETEROCYCLIC COMPOUNDS
HISTONES
HYDROCARBONS
INVERTEBRATES
MAMMALS
MICE
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENANTHROLINES
PROGRESS REPORT
PROTEINS
RODENTS
SEA URCHINS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES