The impact of energy related pollutants on chromosome structure
The sequence preferences of DNA binding by five intercalating chemicals were examined by techniques analogous to chemical DNA sequencing. The intercalators used were 7R, 8S-dihydroxy-9S, 10R-epoxy-7, 8, 9, 10-tetrahydrobenzo(a)pyrene (anti-(+)-BPDE) and its enantiomer, anti-({minus})-BPDE; 8-azido-ethidium, 7-azido-actinomycin D, and bis-(1, 10-phenanthroline)Cu(I). The former four compounds form covalent adducts, some fraction of which are labile and cause DNA chain cleavage upon treatment with hot piperidine. The latter compound causes spontaneous chain cleavage via activated oxygen species generated in the presence of molecular oxygen and a reducing agent. Quantitative mapping data on the DNA cleavages caused by these chemicals were obtained on over 1200 DNA bases in restriction fragments and were statistically analyzed. The sequence preferences of all compounds were best described in terms of base triplets, but bases flanking the preferred triplets also influenced binding in many cases. Although the intercalators differed in degree of sequence selectivity and triplet sequences most preferred, YRY and YGG sequences were generally preferred, especially when the 5'-Y was T, while RRY and oligo-purine/oligo-pyrimidine tracts were generally avoided. Details of individual intercalator preferences and other features of the DNA binding of these compounds are described. 17 refs.
- Research Organization:
- Florida State Univ., Tallahassee, FL (USA). Inst. of Molecular Biophysics
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- FG05-87ER60588
- OSTI ID:
- 5345926
- Report Number(s):
- DOE/ER/60588-3; ON: DE90002541
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLAMINOFLUORENES
ACTINOMYCIN
ADDUCTS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AQUATIC ORGANISMS
AROMATICS
AZAARENES
BENZOPYRENE
CODONS
CONDENSED AROMATICS
DNA ADDUCTS
DNA SEQUENCING
DOCUMENT TYPES
DRUGS
ECHINODERMS
HETEROCYCLIC COMPOUNDS
HISTONES
HYDROCARBONS
INVERTEBRATES
MAMMALS
MICE
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENANTHROLINES
PROGRESS REPORT
PROTEINS
RODENTS
SEA URCHINS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES