Effects of nitrogen dioxide on red blood cells of rats: changes in components of red cell membranes during in vivo exposure to NO/sub 2/

Journal Article · Sun Nov 30 23:00:00 EST 1980 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(80)90074-2· OSTI ID:6538151

Kaya, K; Miura, T; Kubota, K

Red cell membranes, prepared from red blood cells of rats exposed to 4, 10, or 20 ppM nitrogen dioxide (NO/sub 2/) for 1 to 10 days, were examined for evidence of changes in membrane components. Appreciable changes were not found in contents of phospholipid and cholesterol during exposure to 10 ppM NO/sub 2/. By contrast, protein content altered with the time of exposure. Moreover, changes in protein composition were observed by employing sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Twenty-four-hour exposure to NO/sub 2/ at the concentration above 10 ppM resulted in a marked increase in the percentage of lysophosphatidylethanolamine (LysoPE) to the total phospholipids. The prolonged exposure to 10 ppM NO/sub 2/ gave rise to a further increase in LysoPE, whereas the percentage of phosphatidylethanolamine (PE) showed a gradual decrease. A 1-day exposure to 4.0 ppM NO/sub 2/ also caused an increase in sialic acid content and decreases in those of PE and hexose. In addition to contents of these components the percentage of LysoPE increased 5 days after exposure and the elevated values were maintained up to the end of exposure period. These results demonstrate that red blood cells in circulation exhibit different membrane properties in terms of lipid and carbohydrate composition during 10 days of exposure to 4.0 ppM NO/sub 2/.

• Research Organization: National Inst. for Environmental Studies, Tsukuba, Japan
• OSTI ID: 6538151
• Journal Information: Environ. Res.; (United States), Journal Name: Environ. Res.; (United States) Vol. 23:2; ISSN ENVRA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CHALCOGENIDES
ERYTHROCYTES
ESTERS
IN VIVO
LIPIDS
MAMMALS
MATERIALS
MEMBRANES
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PATHOLOGICAL CHANGES
PHOSPHOLIPIDS
PROTEINS
RATS
RODENTS
SENSITIVITY
TIME DEPENDENCE
TOXICITY
VERTEBRATES