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Further studies of the induction and intracellular repair of DNA strand breaks using intranuclear SV40 as a test system

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3577389· OSTI ID:6537481
;

We have previously published the techniques and preliminary results of an SV40 viral probe assay for gamma-radiation-induced single- and double-strand DNA breaks and their intracellular repair in higher cells. Those experiments with SV40 infected CV-1 monkey kidney cells suggested that this assay technique demonstrates slow but extensive intracellular repair of single-strand breaks (SSB), and possible early repair of double-strand breaks (DSB), followed by later induction of DSB. Following up on these early observations, many additional infection-incubation experiments have now been performed with both human and simian cells. Analysis of data from these experiments involving up to 6 h of postinfection intranuclear incubation shows the same distribution of strand break damage in incubated and unincubated samples. This implies that under these experimental conditions there is neither intracellular repair nor further production of SSB or DSB in intranuclear viral DNA. We have evidence which suggests that this lack of repair or degradation occurs because the bulk of intranuclear SV40 DNA is relatively inaccessible to host cell enzymes.

Research Organization:
Univ. of Pennsylvania School of Medicine, Philadelphia (USA)
OSTI ID:
6537481
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 116:3; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CONNECTIVE TISSUE CELLS
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
KIDNEYS
MAMMALS
MICROORGANISMS
MONKEYS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
SIMIAN VIRUS
SOMATIC CELLS
STRAND BREAKS
VERTEBRATES
VIRUSES