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Papovavirus probes for DNA repair in human cells: Final report, August 15, 1983-May 15, 1987

Technical Report ·
OSTI ID:6478989

Extracellularly gamma irradiated simian virus 40 (SV40) was used as a model system to study the induction and intracellular repair of DSB (double-strand DNA breaks) and SSB (single-strand DNA breaks) in higher cells. The optimal experimental conditions for this approach were determined and the comparability of radiation dose-response data were compared with those obtained by other techniques. Experiments in progress suggest that limited accessibility of heavily irradiated viral DNA to host cell enzymes leads to variable rates of repair of DSB or SSB. The effects of DNA configuration and molecular environment on the radiation induction of DSB, using SV40 as a model system are addressed. Results indicate that the intranuclear environment is highly protective against DSB induced by ionizing radiation. 9 refs., 11 figs., 3 tabs.

Research Organization:
Pennsylvania Univ., Philadelphia (USA). Dept. of Radiation Therapy
DOE Contract Number:
AC02-83ER60163
OSTI ID:
6478989
Report Number(s):
DOE/ER/60163-3; ON: DE87012461
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
550701 -- Microbiology-- Tracer Techniques
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DAYS LIVING RADIOISOTOPES
DNA REPAIR
DNA-ASE
DOSE-RESPONSE RELATIONSHIPS
ENDONUCLEASES
ENZYMES
ESTERASES
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIATION EFFECTS
RADIOISOTOPES
RECOVERY
REPAIR
SIMIAN VIRUS
STRAND BREAKS
VIRUSES